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Difference between revisions of "Molecular pathology"
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→Tabular comparisons: tweak table
(→Tabular comparisons: more) |
(→Tabular comparisons: tweak table) |
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| DNA sequencing | | DNA sequencing | ||
| PCR, sequencing machine | | PCR, sequencing machine | ||
| any change in genome; does not account for post-transcriptional changes (one cannot definitively infer protein level change) | | any (small) DNA change in the genome; does not account for post-transcriptional changes (one cannot definitively infer protein level change) | ||
| $$$$ | | $$$$ | ||
| gold standard | | gold standard; will not detect large scale changes unless the break points/fusion regions are sequenced | ||
|- | |- | ||
| RNA sequencing | | RNA sequencing | ||
| Line 30: | Line 30: | ||
| any change in the mRNA (post-splicing); useful for infering protein level changes | | any change in the mRNA (post-splicing); useful for infering protein level changes | ||
| $$$ | | $$$ | ||
| less costly than DNA sequencing | | less costly than DNA sequencing - as extrons are not sequenced | ||
|- | |- | ||
| Restriction fragment length polymorphism (RFLP) | | Restriction fragment length polymorphism (RFLP) | ||
| Line 81: | Line 81: | ||
| large scale changes (fusions, deletions, translocations) | | large scale changes (fusions, deletions, translocations) | ||
| $$$ | | $$$ | ||
| | | gives the "big picture" view of the gene | ||
|} | |} | ||