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Difference between revisions of "Molecular pathology"
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==Molecular== | ==Molecular== | ||
General: | General: | ||
*Very small changes. | *Very small changes - submicroscopic. | ||
*Sequence data. | |||
Techniques: | Techniques: | ||
*DNA sequencing - | *DNA sequencing. | ||
**Real time-PCR, [[AKA]] real time-quantitative PCR (RQ-PCR). | |||
*RNA sequencing. | *RNA sequencing. | ||
**May be examined after reverse transcription (RNA -> DNA), i.e. RT-PCR. | |||
*Southern blot. | *Southern blot. | ||
**Analysis of proteins. | |||
===Tests=== | ===Tests=== | ||
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*Large changes (chromosomal). | *Large changes (chromosomal). | ||
**Maximum resolution 3-4 megabase pairs (3-4 million base pairs); may be less - dependent on band density.<ref>{{Ref WMSP|695}}</ref> | **Maximum resolution 3-4 megabase pairs (3-4 million base pairs); may be less - dependent on band density.<ref>{{Ref WMSP|695}}</ref> | ||
*Morphologic data. | |||
Techniques: | Techniques: | ||