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Difference between revisions of "Molecular pathology"
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→Tabular comparisons: +another table
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===Tabular comparisons=== | ===Tabular comparisons=== | ||
====Overview==== | |||
A simplified overview of molecular pathology: | |||
{| class="wikitable sortable" style="margin-left:auto;margin-right:auto" | |||
! Name of technique | |||
! Advantages | |||
! Disadvantages | |||
|- | |||
| in situ hybridization (ISH) | |||
| good way to find gene losses and duplications (one colour) and gene splits and fusions (two colours); intermediate resolution - better resolution than karyotyping for specific target | |||
| target specific (if the target is wrong no information is gained ''or'' one is mislead by negative result); NOT good for "going on a fishing expedition", i.e. looking for changes when one doesn't quite know what is wrong | |||
|- | |||
| karyotyping | |||
| finds large scale gains, losses and rearrangements; good for "going on a fishing expedition", i.e. looking for changes when one doesn't quite know what is wrong | |||
| low resolution (completely misses small scale changes) | |||
|- | |||
| PCR + sequencing ''or'' enzyme digestion and electrophoresis | |||
| high resolution (can find very small changes, e.g. base pair substitutions) | |||
| expensive; thus, limited to small regions; sequencing is the gold standard but very costly; enzyme digestion and electrophoresis is a compromise of sorts where one needs to know something about the expected abnormality | |||
|} | |||
====PCR-based techniques==== | ====PCR-based techniques==== | ||
A comparison of common molecular techniques: | A comparison of common molecular techniques: | ||