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Difference between revisions of "Molecular pathology"
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→Tabular comparisons: more
(→Tabular comparisons: more) |
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| Line 16: | Line 16: | ||
! Name of technique | ! Name of technique | ||
! Key elements | ! Key elements | ||
! Type of change | ! Type of change detected | ||
! Cost | ! Cost | ||
! Other | ! Other | ||
| Line 41: | Line 41: | ||
| gel electrophoresis, hybridization probe with label | | gel electrophoresis, hybridization probe with label | ||
| useful for finding a specific known change, quantifying gene copy number | | useful for finding a specific known change, quantifying gene copy number | ||
| $$$ | |||
| | |||
|- | |||
| Amplification-refractory mutation system (ARMS) | |||
| PCR with mutation-specific primer, gel electrophoresis | |||
| useful for finding a specific known change | |||
| $$ | |||
| primers can be thought of as a hybridization probe; no mutation-specific hybridization (of primer) --> no PCR product | |||
|} | |||
A comparison of ISH and karyotyping: | |||
{| class="wikitable sortable" style="margin-left:auto;margin-right:auto" | |||
! Name of technique | |||
! Key elements | |||
! Type of change detected | |||
! Cost | |||
! Other | |||
|- | |||
| ISH break apart probe | |||
| probes label two parts of a (normal) gene; the two markers straddle (common) break points | |||
| gene fragmentation consistent with translocation; one may find: loss of part of the gene, gene duplication | |||
| $$$ | |||
| can detect translocations - without knowing the specific fusion product | |||
|- | |||
| ISH fusion probe | |||
| probes label different genes (that are not adjacent) | |||
| translocation involving the two genes labeled; one may find: gene duplication, loss of a gene) | |||
| $$$ | |||
| can detect one specific translocation | |||
|- | |||
| ISH one colour | |||
| probe label one region (gene) | |||
| gene duplication, loss of a gene | |||
| $$$ | |||
| | |||
|- | |||
| Karyotyping | |||
| metaphase nuclei | |||
| large scale changes (fusions, deletions, translocations) | |||
| $$$ | | $$$ | ||
| | | | ||