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Difference between revisions of "Molecular Pathology Rotation Notes"
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Molecular Pathology Rotation Notes (view source)
Revision as of 00:23, 28 July 2015
, 00:23, 28 July 2015no edit summary
(Created page with "===UNIT 1=== {{hidden|List three differences between DNA and RNA.|DNA (double stranded, thymine, deoxyribose, more stable; RNA single stranded, ribose, uracil}} {{hidden...") |
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{{hidden|Describe briefly Sanger sequencing.|<center>[[DIdeoxynucleotides are used in a mix with deoxynucleotides, the Di*** terminate the chain, and so you get all possible lengths of chains so then you put them all in order and you can read (based on weight) which one is at each position]]</center>}} | {{hidden|Describe briefly Sanger sequencing.|<center>[[DIdeoxynucleotides are used in a mix with deoxynucleotides, the Di*** terminate the chain, and so you get all possible lengths of chains so then you put them all in order and you can read (based on weight) which one is at each position]]</center>}} | ||
{{hidden|Describe briefly how Taqman automated genotyping is used for allele detection. |<center>[[ | {{hidden|Describe briefly how Taqman automated genotyping is used for allele detection. |<center>[[]]</center>}} | ||
{{hidden|How are DNA microarrays used to identify drug disposition or responses?|<center>[[ | {{hidden|How are DNA microarrays used to identify drug disposition or responses?|<center>[[]]</center>}} | ||
===UNIT 4=== | ===UNIT 4=== | ||
{{hidden|Describe the procedure for submitting FFPE slides for KRAS for colorectal cancer.|<center>[[ | {{hidden|Describe the procedure for submitting FFPE slides for KRAS for colorectal cancer.|<center>[[]]</center>}} | ||
{{hidden|Compare and contrast uniplex versus multiplex genotyping. |<center>[[ | {{hidden|Compare and contrast uniplex versus multiplex genotyping. |<center>[[]]</center>}} | ||
{{hidden|Compare and contrast conventional vs massively parallel sequencing. |<center>[[ | {{hidden|Compare and contrast conventional vs massively parallel sequencing. |<center>[[]]</center>}} | ||
{{hidden|What is multiplex ligation-dependent ligation (MLPA)?|<center>[[ | {{hidden|What is multiplex ligation-dependent ligation (MLPA)?|<center>[[]]</center>}} | ||
{{hidden|What is fragment analysis?|<center>[[ | {{hidden|What is fragment analysis?|<center>[[]]</center>}} | ||
{{hidden|Compare and contrast RT-PCR vs qRTPCR.|<center>[[ | {{hidden|Compare and contrast RT-PCR vs qRTPCR.|<center>[[]]</center>}} | ||
{{hidden|What is MSI?|<center>[[ | {{hidden|What is MSI?|<center>[[]]</center>}} | ||
{{hidden|What is methylation analysis?|<center>[[ | {{hidden|What is methylation analysis?|<center>[[]]</center>}} | ||
===UNIT 5=== | ===UNIT 5=== | ||
{{hidden|What are the four test features required to be documented by the CLIA?|<center>[[ | {{hidden|What are the four test features required to be documented by the CLIA?|<center>[[]]</center>}} | ||
{{hidden|What are "in vitro diagnostics" vs "laboratory developed tests"?|<center>[[Microsatellite instability]]</center>}} | {{hidden|What are "in vitro diagnostics" vs "laboratory developed tests"?|<center>[[Microsatellite instability]]</center>}} | ||