Difference between revisions of "Microphotography"

From Libre Pathology
Jump to navigation Jump to search
(nl update)
Line 1: Line 1:
[[Image:Teratoma 2 low mag.jpg|thumb|250px|[[Micrograph]] of a [[teratoma]]. [[H&E stain]].]]
The article deals with '''microphotography''', i.e. creating '''microphotos'''.  
The article deals with '''microphotography''', i.e. creating '''microphotos'''.  



Revision as of 05:33, 9 October 2014

The article deals with microphotography, i.e. creating microphotos.

Taking the picture

Camera settings

Exposure compensation:

  • None.

White balance:

  • Normal.

Focusing

Setting live view:

  • Rench (II).
    • Live view function settings.
      • Live View shoot.
  • Press "Set" button to open the shutter.

Use the zoom button (on the camera): press twice to digitally magnify 10x.

  • Focus microscope as usual (@ 10x digital magnification).

Note:

  • Post-processing will not fix a blurry image. They way to get sharp images is take sharp pictures!

Composition

  • The centre of interest, usually, should be at the centre.
    • An artsy look can be achieved by making use of the rule-of-thirds.
      • Rule-of-thirds: centre of interest is at one of the four intersects of the imaginary lines that divide the image into thirds.

Post-processing

GIMP scripts:

  • White-balance.
  • Shadows & highlight.

White balance

  • May be done with:
    • White balance plugin.[1]
    • Curves function (in GIMP).

Curves function (in GIMP)

  • Adjust magnitude, then blue, then green -- red should be last.
    • Low magnification images tend toward "too pink" with the white-balance script.

White balance plugin (script)

Procedure:

  1. Select
    • Filters.
      • Colors.
        • White balance.
  2. Use eye dropper to select what should be white on the image.
  3. Set to "background color".

Edge effects/vignetting

The lens distortion/lighting effects at edge of image are known as: "barrel distortion" and "vignetting".

Notes:

De-vignetting (GIMP)

  1. Take picture as one wants.
  2. Remove slide and shoot the background.

In GIMP:

  1. Load image.
  2. Load background as a layer ("Open as layer... ").
  3. Make background into an "overlay" in Layers dialog box.
  4. Invert background.
  5. Save as jpg (merge with background).

Stitching

  • A technique to increase the field of view.

Software:

Image:

Focus stacking

Software:

Image:

Dirt and defects

  • It is best to clean the slide.
    • Photons are free... time is not.
  • Dirt and defects can be removed with the clone tool in GIMP.

Sharpening

  • Images should be sharpened to enhance edges.
    • This is particularly important if the image is projected on a large screen and/or enlarged.
    • Over-sharpening makes images look like caricatures.
  • Sharpening should be the last step in post-processing.

See also

References

  1. URL: http://registry.gimp.org/node/72. Accessed on: 26 July 2010.

External links