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Difference between revisions of "Microphotography"
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=Taking the picture= | =Taking the picture= | ||
== | ==The keys to good pictures== | ||
*Spend time on set-up. | *Spend time on set-up. | ||
**Clean the slide. | **Clean the slide. | ||
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**Put normal beside pathologic - so one has a reference point. | **Put normal beside pathologic - so one has a reference point. | ||
***Transititions are usually interesting. | ***Transititions are usually interesting. | ||
** | **Where to put the centre of interest: | ||
***Truly beautiful: use the ''rule of thirds'' - see ''[[Microphotography#Composition|composition section]]''. | |||
***Functional: centre of interest in the centre. | |||
*Focus. | *Focus. | ||
**Sharpness of nuclear membrane and detail in the cytoplasm. | **Sharpness of nuclear membrane and detail in the cytoplasm. | ||
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*[[De-vignetting]]. | *[[De-vignetting]]. | ||
*Pictures at different magnification. | *Pictures at different magnification. | ||
** | **Sets are usually better than one. | ||
**Many entities have high and low power features. | **Many entities have high and low power features. | ||
*** | ***It is often impossible to capture them with one picture. | ||
==Camera settings== | ==Camera settings== | ||
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==Composition== | ==Composition== | ||
*The centre of interest | [[Image:Rivertree thirds md.gif|thumb|150px|A picture that uses the ''rule of thirds''. (WC)]] | ||
Functional pictures that tell a story and are easy to understand: | |||
*The centre of interest at the centre. | |||
An artsy look can be achieved by making use of the [[Wikipedia:Rule_of_thirds|rule of thirds]]. | |||
*Rule of thirds: centre of interest is at one of the four intersects of the imaginary lines that divide the image into thirds. | |||
=Post-processing= | =Post-processing= | ||
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Some random notes: | Some random notes: | ||
*Software for microscope cameras usually have de-vignetting tools. The tools go by different names and usually entail taking a background image. Ideally, one should shoot the background image at the same magnification as the (primary) image. | |||
*Vignetting is usually worse at lower magnification. | |||
Random links: | |||
*[http://www.gimp.org/release-notes/gimp-2.4.html http://www.gimp.org/release-notes/gimp-2.4.html]. | *[http://www.gimp.org/release-notes/gimp-2.4.html http://www.gimp.org/release-notes/gimp-2.4.html]. | ||
*[http://www.gimp.org/release-notes/gimp-2.4-videos.html http://www.gimp.org/release-notes/gimp-2.4-videos.html]. | *[http://www.gimp.org/release-notes/gimp-2.4-videos.html http://www.gimp.org/release-notes/gimp-2.4-videos.html]. | ||
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**Over-sharpening makes images look like caricatures. | **Over-sharpening makes images look like caricatures. | ||
*Sharpening should be the last step in post-processing. | *Sharpening should be the last step in post-processing. | ||
==Animation== | |||
[[Image:Squamous carcinoma - lung FNA -- high and very high mag - animation.gif|thumb|right|An animated GIF showing [[squamous carcinoma of the lung]]. (WC)]] | |||
*GIMP has a [https://www.gimp.org/tutorials/Simple_Animations/ nice tutorial (gimp.org)] on how it can be done. | |||
*Animations are particularly useful for [[cytopathology]]. | |||
=See also= | =See also= | ||
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*[[Digital ISO]]. | *[[Digital ISO]]. | ||
*[[Micrographs]]. | *[[Micrographs]]. | ||
*[[Image annotation]]. | |||
*[[Micrograph wishlist]]. | |||
=References= | =References= | ||