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Difference between revisions of "Microphotography"
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=Taking the picture= | =Taking the picture= | ||
==The keys to good pictures== | |||
*Spend time on set-up. | |||
**Clean the slide. | |||
**Look for the pristine areas without artifacts, e.g. folds. | |||
*Composition. | |||
**Put normal beside pathologic - so one has a reference point. | |||
***Transititions are usually interesting. | |||
**Where to put the centre of interest: | |||
***Truly beautiful: use the ''rule of thirds'' - see ''[[Microphotography#Composition|composition section]]''. | |||
***Functional: centre of interest in the centre. | |||
*Focus. | |||
**Sharpness of nuclear membrane and detail in the cytoplasm. | |||
*White balance. | |||
*[[De-vignetting]]. | |||
*Pictures at different magnification. | |||
**Sets are usually better than one. | |||
**Many entities have high and low power features. | |||
***It is often impossible to capture them with one picture. | |||
==Camera settings== | ==Camera settings== | ||
Exposure compensation: | Exposure compensation: | ||
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==Composition== | ==Composition== | ||
*The centre of interest | [[Image:Rivertree thirds md.gif|thumb|150px|A picture that uses the ''rule of thirds''. (WC)]] | ||
Functional pictures that tell a story and are easy to understand: | |||
*The centre of interest at the centre. | |||
An artsy look can be achieved by making use of the [[Wikipedia:Rule_of_thirds|rule of thirds]]. | |||
*Rule of thirds: centre of interest is at one of the four intersects of the imaginary lines that divide the image into thirds. | |||
=Post-processing= | =Post-processing= | ||
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==Vignetting== | ==Vignetting== | ||
The | Defintion: | ||
*The edge of images are darker than the centre. | |||
Microscope configuration: | |||
*[[Köhler illumination]]. | |||
*Ensure diaphragm is open. | |||
Some random notes: | |||
*Software for microscope cameras usually have de-vignetting tools. The tools go by different names and usually entail taking a background image. Ideally, one should shoot the background image at the same magnification as the (primary) image. | |||
*Vignetting is usually worse at lower magnification. | |||
Random links: | |||
*[http://www.gimp.org/release-notes/gimp-2.4.html http://www.gimp.org/release-notes/gimp-2.4.html]. | *[http://www.gimp.org/release-notes/gimp-2.4.html http://www.gimp.org/release-notes/gimp-2.4.html]. | ||
*[http://www.gimp.org/release-notes/gimp-2.4-videos.html http://www.gimp.org/release-notes/gimp-2.4-videos.html]. | *[http://www.gimp.org/release-notes/gimp-2.4-videos.html http://www.gimp.org/release-notes/gimp-2.4-videos.html]. | ||
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*[http://hugin.sourceforge.net/ Hugin (sourceforge.net)]. | *[http://hugin.sourceforge.net/ Hugin (sourceforge.net)]. | ||
Image: | ===Images=== | ||
<gallery> | |||
Image:Londonpanorama.jpg | Example of stitching. (WC) | |||
Image:Enterobius - very low mag.jpg | Another example of stitching. (WC) | |||
</gallery> | |||
==Focus stacking== | ==Focus stacking== | ||
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**Over-sharpening makes images look like caricatures. | **Over-sharpening makes images look like caricatures. | ||
*Sharpening should be the last step in post-processing. | *Sharpening should be the last step in post-processing. | ||
==Animation== | |||
[[Image:Squamous carcinoma - lung FNA -- high and very high mag - animation.gif|thumb|right|An animated GIF showing [[squamous carcinoma of the lung]]. (WC)]] | |||
*GIMP has a [https://www.gimp.org/tutorials/Simple_Animations/ nice tutorial (gimp.org)] on how it can be done. | |||
*Animations are particularly useful for [[cytopathology]]. | |||
=See also= | =See also= | ||
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*[[Digital ISO]]. | *[[Digital ISO]]. | ||
*[[Micrographs]]. | *[[Micrographs]]. | ||
*[[Image annotation]]. | |||
*[[Micrograph wishlist]]. | |||
=References= | =References= | ||