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Difference between revisions of "Immunohistochemical staining"
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Immunohistochemical staining (view source)
Revision as of 12:16, 8 October 2013
, 12:16, 8 October 2013→Interpretation
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#*Patchy. | #*Patchy. | ||
#*Diffuse. | #*Diffuse. | ||
#Where it stains the various | #Where it stains the various tissues: | ||
#*Nucleus. | #*Nucleus. | ||
#*Cytoplasm. | #*Cytoplasm. | ||
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#*A combination of the above. | #*A combination of the above. | ||
Generally, interpretations | Generally, interpretations can be subjective, and this is especially true when the staining is weak and focal. In other words, "... your weak [positive] stain might be somebody else’s negative."<ref>URL: [http://bitesizebio.com/articles/immunohistochemistry-getting-the-stain-you-want/ http://bitesizebio.com/articles/immunohistochemistry-getting-the-stain-you-want/]. Accessed on: 1 September 2012.</ref> | ||
The cynical might say it is unwritten rule that: "... if the stain is weak and focal it can be anything you want to make it -- positive or negative -- so it fits perfectly with your diagnosis!" | The cynical might say it is unwritten rule that: "... if the stain is weak and focal it can be anything you want to make it -- positive or negative -- so it fits perfectly with your diagnosis!" | ||
In cases where the morphology is unclear it is judicious to have two or more | In cases where the morphology is unclear, it is judicious to have two or more immunostains that support the diagnosis, and negative stains for important entities in the differential diagnosis. | ||
==General (malignant) differential diagnosis== | ==General (malignant) differential diagnosis== | ||
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