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Difference between revisions of "Immunohistochemical staining"
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{{familytree/end}} | {{familytree/end}} | ||
*Carcinoma | *Carcinoma. | ||
**AE1/AE3 - pankeratin. | **AE1/AE3 - pankeratin. | ||
*Sarcoma | *Sarcoma. | ||
**vimentin. | **vimentin. | ||
***many pathologists think this stain is totally useless | ***many pathologists think this stain is totally useless | ||
*Germ cell tumours | *Germ cell tumours. | ||
**PLAP (placental-like alkaline phosphatase) - not very sensitive.<ref name=pmid18045648>{{cite journal |author=Iczkowski KA, Butler SL, Shanks JH, ''et al'' |title=Trials of new germ cell immunohistochemical stains in 93 extragonadal and metastatic germ cell tumors |journal=Hum. Pathol. |volume=39 |issue=2 |pages=275-81 |year=2008 |month=February |pmid=18045648 |doi=10.1016/j.humpath.2007.07.002 |url=}}</ref> | **PLAP (placental-like alkaline phosphatase) - not very sensitive.<ref name=pmid18045648>{{cite journal |author=Iczkowski KA, Butler SL, Shanks JH, ''et al'' |title=Trials of new germ cell immunohistochemical stains in 93 extragonadal and metastatic germ cell tumors |journal=Hum. Pathol. |volume=39 |issue=2 |pages=275-81 |year=2008 |month=February |pmid=18045648 |doi=10.1016/j.humpath.2007.07.002 |url=}}</ref> | ||
*Neuroendocrine carcinoma | *Neuroendocrine carcinoma | ||
| Line 22: | Line 22: | ||
**synaptophysin. | **synaptophysin. | ||
**CD56. | **CD56. | ||
*Melanoma | *Melanoma. | ||
**S-100, HMB-45, Melan A (MART-1). | **S-100, HMB-45, Melan A (MART-1). | ||
*Lymphoma | *Lymphoma. | ||
**CD45 (common leukocyte antigen). | **CD45 (common leukocyte antigen). | ||
| Line 38: | Line 38: | ||
==Organ specific== | ==Organ specific== | ||
===Thyroid=== | ===Thyroid=== | ||
*TTF-1 (thyroid transcription factor-1) | *TTF-1 (thyroid transcription factor-1). | ||
**Very good for breast vs. lung.<ref name=pmid18318581>{{cite journal |author=Jagirdar J |title=Application of immunohistochemistry to the diagnosis of primary and metastatic carcinoma to the lung |journal=Arch. Pathol. Lab. Med. |volume=132 |issue=3 |pages=384–96 |year=2008 |month=March |pmid=18318581 |doi= |url=http://journals.allenpress.com/jrnlserv/?request=get-abstract&issn=0003-9985&volume=132&page=384}}</ref> | **Very good for breast vs. lung.<ref name=pmid18318581>{{cite journal |author=Jagirdar J |title=Application of immunohistochemistry to the diagnosis of primary and metastatic carcinoma to the lung |journal=Arch. Pathol. Lab. Med. |volume=132 |issue=3 |pages=384–96 |year=2008 |month=March |pmid=18318581 |doi= |url=http://journals.allenpress.com/jrnlserv/?request=get-abstract&issn=0003-9985&volume=132&page=384}}</ref> | ||
***Negative in squamous cell carcinoma of the lung (as with CK7 & CK20), though HMWK is usually positive. | ***Negative in squamous cell carcinoma of the lung (as with CK7 & CK20), though HMWK is usually positive. | ||
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===Small bowel=== | ===Small bowel=== | ||
*CDX2. | *CDX2. | ||
* | *Villin. | ||
===Kidney=== | ===Kidney=== | ||
[[Renal cell carcinoma]] | [[Renal cell carcinoma]] | ||
*RCC, EMA, CD10. | *RCC, EMA, CD10. | ||
*CK7 | *CK7 -ve in clear cell RCC. | ||
*[[AMACR]] | *[[AMACR]] +ve in papillary RCC. | ||
*D2-40 | *D2-40 +ve in ChRCC. | ||
Xanthogranulomatous pyelonephritis | Xanthogranulomatous pyelonephritis | ||
| Line 114: | Line 114: | ||
==One organ vs. another== | ==One organ vs. another== | ||
===Cervix vs. uterus=== | ===Cervix vs. uterus=== | ||
*Cervix (typically): CEA+,<ref name=pmid12647218>{{cite journal |author=Alkushi A, Irving J, Hsu F, ''et al.'' |title=Immunoprofile of cervical and endometrial adenocarcinomas using a tissue microarray |journal=Virchows Arch. |volume=442 |issue=3 |pages=271-7 |year=2003 |month=March |pmid=12647218 |doi=10.1007/s00428-002-0752-4 |url=}}</ref> p16+ | *Cervix (typically): CEA +ve,<ref name=pmid12647218>{{cite journal |author=Alkushi A, Irving J, Hsu F, ''et al.'' |title=Immunoprofile of cervical and endometrial adenocarcinomas using a tissue microarray |journal=Virchows Arch. |volume=442 |issue=3 |pages=271-7 |year=2003 |month=March |pmid=12647218 |doi=10.1007/s00428-002-0752-4 |url=}}</ref> p16 +ve. | ||
** ... and ER-, PR-, vimentin- | ** ... and ER -ve, PR -ve, vimentin -ve. | ||
*Uterus (typically): vimentin+, ER+, PR+<ref>[http://www.nature.com/modpathol/journal/v19/n8/full/3800620a.html http://www.nature.com/modpathol/journal/v19/n8/full/3800620a.html]</ref> | *Uterus (typically): vimentin +ve, ER +ve, PR +ve.<ref>URL: [http://www.nature.com/modpathol/journal/v19/n8/full/3800620a.html http://www.nature.com/modpathol/journal/v19/n8/full/3800620a.html]</ref> | ||
** ... and CEA-, p16- | ** ... and CEA -ve, p16 -ve. | ||
Additional Ref.:<ref>LAE 15 Jan 2009.</ref> | Additional Ref.:<ref>LAE 15 Jan 2009.</ref> | ||