Difference between revisions of "Fontana-Masson stain"

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#redirect [[Stains#Fontana-Masson_stain]]
'''Fontana-Masson stain''' is a special stain that marks the pigment melanin.
 
It is also known as ''Masson-Fontana stain'',<ref name=pmid16081962>{{Cite journal  | last1 = Gaitanis | first1 = G. | last2 = Chasapi | first2 = V. | last3 = Velegraki | first3 = A. | title = Novel application of the masson-fontana stain for demonstrating Malassezia species melanin-like pigment production in vitro and in clinical specimens. | journal = J Clin Microbiol | volume = 43 | issue = 8 | pages = 4147-51 | month = Aug | year = 2005 | doi = 10.1128/JCM.43.8.4147-4151.2005 | PMID = 16081962 }}</ref> ''Fontana-Masson stain for melanin'', ''melanin stain''.
*A type of silver stain.
 
==Stains==
#Melanin.
#"Argentaffin granules" of the digestive tract.
#Pigment deposition due to minocycline treatment.<ref name=pmid15605966>{{Cite journal  | last1 = Patterson | first1 = JW. | last2 = Wilson | first2 = B. | last3 = Wick | first3 = MR. | last4 = Heath | first4 = C. | title = Hyperpigmented scar due to minocycline therapy. | journal = Cutis | volume = 74 | issue = 5 | pages = 293-8 | month = Nov | year = 2004 | doi =  | PMID = 15605966 }}</ref>
 
==Use==
*Stain for melanin.
**Displays melanin - whether it be in melanocytes, keratinocytes or melanophages.
**IHC stains, i.e. ''Melan A, SOX10 or MITF'' are preferable for displaying ''melanocytes''.
**Used to differentiate brown pigments (lipofuscin, hemosiderin, melanin).<ref>URL: [http://education.vetmed.vt.edu/curriculum/VM8054/labs/Lab2/Examples/exfontana.htm http://education.vetmed.vt.edu/curriculum/VM8054/labs/Lab2/Examples/exfontana.htm]. Accessed on: 5 May 2010.</ref>
**Used to document Minocycline type II drug induced pigment deposition
***Minocycline pigment, Type II will stain with both the Fontana-Masson stain AND the Perls iron stain.<ref name=pmid19595269>{{cite journal |author=Geria AN, Tajirian AL, Kihiczak G, Schwartz RA |title=Minocycline-induced skin pigmentation: an update |journal=Acta Dermatovenerol Croat |volume=17 |issue=2 |pages=123–6 |year=2009 |pmid=19595269 |doi= |url=}}</ref>
**Used in the differential diagnosis of hypomelanosis
***Idiopathic hypomelanosis will demonstrate ONLY loss of melanin with the Fontana-Masson stain - melanocytes will not be absent with the Melan A stain.
***Vitiligo will show loss of melanin AND loss of melanocytes with a Melan A stain.
Image:
*[http://education.vetmed.vt.edu/curriculum/VM8054/labs/Lab2/Examples/exfontana.htm Fontana-Masson stain (vetmed.vt.edu)].
 
==See also==
*[[Stains]].
 
==References==
{{Reflist|1}}


[[Category:Stains]]
[[Category:Stains]]

Revision as of 19:16, 13 April 2016

Fontana-Masson stain is a special stain that marks the pigment melanin.

It is also known as Masson-Fontana stain,[1] Fontana-Masson stain for melanin, melanin stain.

  • A type of silver stain.

Stains

  1. Melanin.
  2. "Argentaffin granules" of the digestive tract.
  3. Pigment deposition due to minocycline treatment.[2]

Use

  • Stain for melanin.
    • Displays melanin - whether it be in melanocytes, keratinocytes or melanophages.
    • IHC stains, i.e. Melan A, SOX10 or MITF are preferable for displaying melanocytes.
    • Used to differentiate brown pigments (lipofuscin, hemosiderin, melanin).[3]
    • Used to document Minocycline type II drug induced pigment deposition
      • Minocycline pigment, Type II will stain with both the Fontana-Masson stain AND the Perls iron stain.[4]
    • Used in the differential diagnosis of hypomelanosis
      • Idiopathic hypomelanosis will demonstrate ONLY loss of melanin with the Fontana-Masson stain - melanocytes will not be absent with the Melan A stain.
      • Vitiligo will show loss of melanin AND loss of melanocytes with a Melan A stain.

Image:

See also

References

  1. ↑ Gaitanis, G.; Chasapi, V.; Velegraki, A. (Aug 2005). "Novel application of the masson-fontana stain for demonstrating Malassezia species melanin-like pigment production in vitro and in clinical specimens.". J Clin Microbiol 43 (8): 4147-51. doi:10.1128/JCM.43.8.4147-4151.2005. PMID 16081962.
  2. ↑ Patterson, JW.; Wilson, B.; Wick, MR.; Heath, C. (Nov 2004). "Hyperpigmented scar due to minocycline therapy.". Cutis 74 (5): 293-8. PMID 15605966.
  3. ↑ URL: http://education.vetmed.vt.edu/curriculum/VM8054/labs/Lab2/Examples/exfontana.htm. Accessed on: 5 May 2010.
  4. ↑ Geria AN, Tajirian AL, Kihiczak G, Schwartz RA (2009). "Minocycline-induced skin pigmentation: an update". Acta Dermatovenerol Croat 17 (2): 123–6. PMID 19595269.
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