Difference between revisions of "Diffuse large B-cell lymphoma"

From Libre Pathology
Jump to navigation Jump to search
(→‎IHC: wikify)
m (→‎IHC: +molecular section)
Line 39: Line 39:
*CD5 -- T cells.
*CD5 -- T cells.
*Unstained x4.
*Unstained x4.
==Molecular==
*Rearrangements of BCL6.<ref>{{OMIM|109565}}</ref>
**Can be assessed with an [[ISH]] break apart probe.
*Translocation typical of [[follicular lymphoma]]: t(14;18)(q32;q21)/IGH-BCL2.<ref name=pmid18359244>{{cite journal |author=Vitolo U, Ferreri AJ, Montoto S |title=Follicular lymphomas |journal=Crit. Rev. Oncol. Hematol. |volume=66 |issue=3 |pages=248–61 |year=2008 |month=June |pmid=18359244 |doi=10.1016/j.critrevonc.2008.01.014 |url=}}</ref>


==See also==
==See also==

Revision as of 19:41, 24 May 2011

Diffuse large B cell lymphoma, abbreviated DLBCL, is a very common lymphoma.

Microscopic

Features:[1]

  • Large lymphoid cells:
    • 4-5x the diameter of a small lymphocytes.
    • Marked cell-to-cell variation in size and shape.
    • Cytoplasm usually basophilic and moderate in abundance.
    • +/-Prominent nucleoli, may be peripheral and/or multiple.
  • Not follicular - nodular arrangement.

Notes:

  • Large bizarre cells can occasionally mimic Reed-Sternberg cells, seen in Hodgkin lymphoma.

Images:

Differential diagnosis

IHC

"UHN panel"

  • H&E 2 micrometers.
  • CD20 -- B cells.
  • CD10 -- follicular lymphoma.
  • BCL6 -- follicular lymphoma.
  • BCL2 -- follicular lymphoma.
  • MUM1 -- B cells.
  • CD21 -- highlight FDC networks if present.
  • CD23 -- highlight FDC networks if present.
  • MIB1 -- proliferative rate.
  • EBER -- EBV.
  • CD3 -- T cells.
  • CD5 -- T cells.
  • Unstained x4.

Molecular

  • Rearrangements of BCL6.[2]
    • Can be assessed with an ISH break apart probe.
  • Translocation typical of follicular lymphoma: t(14;18)(q32;q21)/IGH-BCL2.[3]

See also

References

  1. Cotran, Ramzi S.; Kumar, Vinay; Fausto, Nelson; Nelso Fausto; Robbins, Stanley L.; Abbas, Abul K. (2005). Robbins and Cotran pathologic basis of disease (7th ed.). St. Louis, Mo: Elsevier Saunders. pp. 676 (???). ISBN 0-7216-0187-1.
  2. Online 'Mendelian Inheritance in Man' (OMIM) 109565
  3. Vitolo U, Ferreri AJ, Montoto S (June 2008). "Follicular lymphomas". Crit. Rev. Oncol. Hematol. 66 (3): 248–61. doi:10.1016/j.critrevonc.2008.01.014. PMID 18359244.