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Difference between revisions of "Carbonic anhydrase 9"
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'''Carbonic anhydrase 9''', abbreviated '''CA9''', is a common [[immunostain]] used in [[genitourinary pathology]]. | '''Carbonic anhydrase 9''', abbreviated '''CA9''' and '''CAIX''', is a common [[immunostain]] used in [[genitourinary pathology]]. | ||
==General== | |||
*Several clones available - clone M75 most common.<ref name=pmid18300814>{{Cite journal | last1 = Al-Ahmadie | first1 = HA. | last2 = Alden | first2 = D. | last3 = Qin | first3 = LX. | last4 = Olgac | first4 = S. | last5 = Fine | first5 = SW. | last6 = Gopalan | first6 = A. | last7 = Russo | first7 = P. | last8 = Motzer | first8 = RJ. | last9 = Reuter | first9 = VE. | title = Carbonic anhydrase IX expression in clear cell renal cell carcinoma: an immunohistochemical study comparing 2 antibodies. | journal = Am J Surg Pathol | volume = 32 | issue = 3 | pages = 377-82 | month = Mar | year = 2008 | doi = 10.1097/PAS.0b013e3181570343 | PMID = 18300814 }}</ref> | |||
==Staining== | |||
===Positive=== | |||
*[[Clear cell renal cell carcinoma]].<ref name=pmid21088149>{{Cite journal | last1 = Genega | first1 = EM. | last2 = Ghebremichael | first2 = M. | last3 = Najarian | first3 = R. | last4 = Fu | first4 = Y. | last5 = Wang | first5 = Y. | last6 = Argani | first6 = P. | last7 = Grisanzio | first7 = C. | last8 = Signoretti | first8 = S. | title = Carbonic anhydrase IX expression in renal neoplasms: correlation with tumor type and grade. | journal = Am J Clin Pathol | volume = 134 | issue = 6 | pages = 873-9 | month = Dec | year = 2010 | doi = 10.1309/AJCPPPR57HNJMSLZ | PMID = 21088149 }}</ref> | |||
**95% +ve with clone NB100-417 and clone M75.<ref name=pmid18300814>{{Cite journal | last1 = Al-Ahmadie | first1 = HA. | last2 = Alden | first2 = D. | last3 = Qin | first3 = LX. | last4 = Olgac | first4 = S. | last5 = Fine | first5 = SW. | last6 = Gopalan | first6 = A. | last7 = Russo | first7 = P. | last8 = Motzer | first8 = RJ. | last9 = Reuter | first9 = VE. | title = Carbonic anhydrase IX expression in clear cell renal cell carcinoma: an immunohistochemical study comparing 2 antibodies. | journal = Am J Surg Pathol | volume = 32 | issue = 3 | pages = 377-82 | month = Mar | year = 2008 | doi = 10.1097/PAS.0b013e3181570343 | PMID = 18300814 }}</ref> | |||
*[[Malignant mesothelioma|Epithelioid mesothelioma]].<ref name=pmid23503645>{{Cite journal | last1 = Ordóñez | first1 = NG. | title = Value of PAX8, PAX2, napsin A, carbonic anhydrase IX, and claudin-4 immunostaining in distinguishing pleural epithelioid mesothelioma from metastatic renal cell carcinoma. | journal = Mod Pathol | volume = 26 | issue = 8 | pages = 1132-43 | month = Aug | year = 2013 | doi = 10.1038/modpathol.2013.34 | PMID = 23503645 }}</ref> | |||
*Benign mesothelium.<ref name=pmid24926090>{{Cite journal | last1 = Ananthanarayanan | first1 = V. | last2 = Tretiakova | first2 = M. | last3 = Husain | first3 = AN. | last4 = Krausz | first4 = T. | last5 = Antic | first5 = T. | title = Carbonic anhydrase IX (CAIX) does not differentiate between benign and malignant mesothelium. | journal = Am J Clin Pathol | volume = 142 | issue = 1 | pages = 82-7 | month = Jul | year = 2014 | doi = 10.1309/AJCP8BJ8CJXUEHZD | PMID = 24926090 }}</ref> | |||
===Negative=== | |||
*[[Xp11.2 translocation carcinoma]].<ref name=pmid21088149/> | |||
*Breast carcinoma.<ref name=pmid24436269>{{Cite journal | last1 = Luong-Player | first1 = A. | last2 = Liu | first2 = H. | last3 = Wang | first3 = HL. | last4 = Lin | first4 = F. | title = Immunohistochemical reevaluation of carbonic anhydrase IX (CA IX) expression in tumors and normal tissues. | journal = Am J Clin Pathol | volume = 141 | issue = 2 | pages = 219-25 | month = Feb | year = 2014 | doi = 10.1309/AJCPVJDS28KNYZLD | PMID = 24436269 }}</ref> ‡ | |||
*[[Seminoma]].<ref name=pmid24436269/> | |||
Note: | |||
*‡ A paper by Furjelová ''et al.'' found different results.<ref name=pmid23816158>{{Cite journal | last1 = Furjelová | first1 = M. | last2 = Kovalská | first2 = M. | last3 = Jurková | first3 = K. | last4 = Horáček | first4 = J. | last5 = Carbolová | first5 = T. | last6 = Adamkov | first6 = M. | title = Carbonic anhydrase IX: a promising diagnostic and prognostic biomarker in breast carcinoma. | journal = Acta Histochem | volume = 116 | issue = 1 | pages = 89-93 | month = Jan | year = 2014 | doi = 10.1016/j.acthis.2013.05.009 | PMID = 23816158 }}</ref> | |||
===Images=== | |||
<gallery> | |||
Image: Carbonic anhydrase 9 -- intermed mag.jpg | CA9 - intermed. mag. (WC) | |||
Image: Carbonic anhydrase 9 - alt -- high mag.jpg | CA9 - high mag. (WC) | |||
Image: Carbonic anhydrase 9 -- very high mag.jpg | CA9 - very high mag. (WC) | |||
</gallery> | |||
==See also== | ==See also== | ||
*[[Immunohistochemistry]]. | *[[Immunohistochemistry]]. | ||
*[[RCC (immunostain)]]. | |||
==References== | ==References== | ||