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Difference between revisions of "Basics"
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→Microscopes
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==Microscopes== | ==Microscopes== | ||
{{main|HPFitis}} | |||
*Pathologists throw around the term ''high power field'' (''HPF''). | *Pathologists throw around the term ''high power field'' (''HPF''). | ||
**"HPF" has no agreed upon definition and, IMHO, should '''''never''''' be used without definition. | **"HPF" has no agreed upon definition and, IMHO, should '''''never''''' be used without a non-ambiguous definition. | ||
''HPF'' generally refers to the area seen with the largest magnification objective (40x), i.e. the field at 400x (as the eye piece magnification is usually 10x). The field size varies significantly from microscope to microscope. | ''HPF'' generally refers to the area seen with the largest magnification objective (40x), i.e. the field at 400x (as the eye piece magnification is usually 10x). The field size varies significantly from microscope to microscope. | ||