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Difference between revisions of "Amebiasis"
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| IHC = | | IHC = | ||
| EM = | | EM = | ||
| Molecular = | | Molecular = PCR testing | ||
| IF = | | IF = | ||
| Gross = | | Gross = | ||
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==Stains== | ==Stains== | ||
*Iron-hematoxylin stain - black.<ref name=pimd22523839>{{Cite journal | last1 = Chacín-Bonilla | first1 = L. | title = [Microscopic diagnosis of amebiasis: an obsolete method but necessary in the developing world]. | journal = Invest Clin | volume = 52 | issue = 4 | pages = 291-4 | month = Dec | year = 2011 | doi = | PMID = 22523839 }}</ref><ref>URL: [http://www.histopathology-india.net/amco.htm http://www.histopathology-india.net/amco.htm]. Accessed on: 29 June 2016.</ref> | *Iron-hematoxylin stain - black.<ref name=pimd22523839>{{Cite journal | last1 = Chacín-Bonilla | first1 = L. | title = [Microscopic diagnosis of amebiasis: an obsolete method but necessary in the developing world]. | journal = Invest Clin | volume = 52 | issue = 4 | pages = 291-4 | month = Dec | year = 2011 | doi = | PMID = 22523839 }}</ref><ref>URL: [http://www.histopathology-india.net/amco.htm http://www.histopathology-india.net/amco.htm]. Accessed on: 29 June 2016.</ref> | ||
*Trichrome stain.<ref name=pmid2454958>{{Cite journal | last1 = Shetty | first1 = N. | last2 = Prabhu | first2 = T. | title = Evaluation of faecal preservation and staining methods in the diagnosis of acute amoebiasis and giardiasis. | journal = J Clin Pathol | volume = 41 | issue = 6 | pages = 694-9 | month = Jun | year = 1988 | doi = | PMID = 2454958 }}</ref><ref>URL: [http://emedicine.medscape.com/article/212029-workup http://emedicine.medscape.com/article/212029-workup]. Accessed on: 29 June 2016.</ref> | |||
==IHC== | ==IHC== | ||
*Entamoeba histolytica +ve.<ref name=pmid1283004>{{Cite journal | last1 = Prasad | first1 = R. | last2 = Tola | first2 = M. | last3 = Bhattacharya | first3 = S. | last4 = Sharma | first4 = MP. | last5 = Bhattacharya | first5 = A. | title = Recognition of Entamoeba histolytica lipophosphoglycan by a strain-specific monoclonal antibody and human immune sera. | journal = Mol Biochem Parasitol | volume = 56 | issue = 2 | pages = 279-87 | month = Dec | year = 1992 | doi = | PMID = 1283004 }}</ref> | *Entamoeba histolytica +ve.<ref name=pmid1283004>{{Cite journal | last1 = Prasad | first1 = R. | last2 = Tola | first2 = M. | last3 = Bhattacharya | first3 = S. | last4 = Sharma | first4 = MP. | last5 = Bhattacharya | first5 = A. | title = Recognition of Entamoeba histolytica lipophosphoglycan by a strain-specific monoclonal antibody and human immune sera. | journal = Mol Biochem Parasitol | volume = 56 | issue = 2 | pages = 279-87 | month = Dec | year = 1992 | doi = | PMID = 1283004 }}</ref> | ||
==Molecular== | |||
*May be identified by [[PCR]] with an appropriate primer.<ref name=pmid24192618>{{Cite journal | last1 = Zeyrek | first1 = FY. | last2 = Turgay | first2 = N. | last3 = Unver | first3 = A. | last4 = Ustün | first4 = S. | last5 = Akarca | first5 = U. | last6 = Töz | first6 = S. | title = Differentiation of Entamoeba histolytica/Entamoeba dispar by the polymerase chain reaction in stool samples of patients with gastrointestinal symptoms in the Sanliurfa Province. | journal = Turkiye Parazitol Derg | volume = 37 | issue = 3 | pages = 174-8 | month = | year = 2013 | doi = 10.5152/tpd.2013.39 | PMID = 24192618 }}</ref> | |||
==See also== | ==See also== | ||