Talk:Urothelium

Dysplasia

URINARY BLADDER LESION, DOME, BIOPSY:
- UROTHELIAL DYSPLASIA, SEE COMMENT.
- BENIGN MUSCULARIS PROPRIA.

COMMENT:
The urothelium's nuclei have mild atypia, as would be seen in a low-grade papillary
urothelial carcinoma. 

Immunostaining is as follows:
p53 - weak to moderate staining of 10% of the urothelium, predominantly basal.
CK20 - focal staining of surface cells (umbrella cells), otherwise negative.
Ki-67 - 20-30% of urothelium, predominantly basal.

Ki-67

• 60x objective... estimated ~21 cells across. Thus, ~350 cells in the field (21*21/4*pi ~ 346).
  • If it is 20 cells... it is 314 cells in the field.
• Field Diameter = 22 mm/60 = 0.366667 mm.
• Average cell size = 367 micrometres/21 ~= 17 micrometres.

Muscularis propria

The tumour infiltrates one large nodular bundle of smooth muscle (~ 1 millimetre). That the thick smooth muscle bundle could represent a tangential cut of muscularis mucosae was considered. Levels were cut.