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Difference between revisions of "Molecular pathology"
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→PCR-based techniques: re-arr.
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====PCR-based techniques==== | ====PCR-based/electrophoresis based techniques==== | ||
A comparison of common molecular techniques: | A comparison of common molecular techniques: | ||
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| value of result depends on RFLP data specific to gene, i.e. knowledge about mutations commonly seen in the gene | | value of result depends on RFLP data specific to gene, i.e. knowledge about mutations commonly seen in the gene | ||
|- | |- | ||
| Amplification-refractory mutation system (ARMS) | | Amplification-refractory mutation system (ARMS) | ||
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| primers can be thought of as a hybridization probe; no mutation-specific hybridization (of primer) --> no PCR product | | primers can be thought of as a hybridization probe; no mutation-specific hybridization (of primer) --> no PCR product | ||
|- | |||
| Southern blot | |||
| gel electrophoresis, hybridization probe with label | |||
| useful for finding a specific known change, quantifying gene copy number | |||
| $$$$ | |||
| -rarely done<br>-does '''not''' use PCR<br>-considered the gold standard for clonality<ref name=pmid10583924>{{Cite journal | last1 = Medeiros | first1 = LJ. | last2 = Carr | first2 = J. | title = Overview of the role of molecular methods in the diagnosis of malignant lymphomas. | journal = Arch Pathol Lab Med | volume = 123 | issue = 12 | pages = 1189-207 | month = Dec | year = 1999 | doi = 10.1043/0003-9985(1999)1231189:OOTROM2.0.CO;2 | PMID = 10583924 }}</ref><br>-most labs consider fresh or frozen tissue a requirement<ref name=pmid11070157>{{Cite journal | last1 = Reinartz | first1 = JJ. | last2 = McCormick | first2 = SR. | last3 = Ikier | first3 = DM. | last4 = Mellgen | first4 = AM. | last5 = Bonham | first5 = SC. | last6 = Strickler | first6 = JG. | last7 = Mendiola | first7 = JR. | title = Immunoglobulin heavy-chain gene rearrangement studies by Southern blot using DNA extracted from formalin-fixed, paraffin-embedded tissue. | journal = Mol Diagn | volume = 5 | issue = 3 | pages = 227-33 | month = Sep | year = 2000 | doi = 10.1054/modi.2000.19808 | PMID = 11070157 }}</ref> | |||
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