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Difference between revisions of "Molecular pathology"
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===Tabular comparisons=== | |||
A comparison of common molecular techniques: | |||
{| class="wikitable sortable" style="margin-left:auto;margin-right:auto" | |||
! Name of technique | |||
! Key elements | |||
! Type of change | |||
! Cost | |||
! Other | |||
|- | |||
| DNA sequencing | |||
| PCR, sequencing machine | |||
| any change in genome; does not account for post-transcriptional changes (one cannot definitively infer protein level change) | |||
| $$$$ | |||
| gold standard | |||
|- | |||
| RNA sequencing | |||
| reverse transcription PCR, sequencing maching | |||
| any change in the mRNA (post-splicing); useful for infering protein level changes | |||
| $$$ | |||
| less costly than DNA sequencing (no extrons) | |||
|- | |||
| Restriction fragment length polymorphism (RFLP) | |||
| PCR, restriction endonuclease digestion, gel electrophoresis | |||
| useful for finding common base pair changes | |||
| $$ | |||
| value of result depends on RFLP data specific to gene, i.e. knowledge about mutations commonly seen in the gene | |||
|- | |||
| Southern blot | |||
| gel electrophoresis, hybridization probe with label | |||
| useful for finding a specific known change, quantifying gene copy number | |||
| $$$ | |||
| | |||
|} | |||
==PCR-based techniques== | ==PCR-based techniques== | ||