Difference between revisions of "O-6-methylguanine-DNA methyltransferase"

MGMT testing in neuropathology is usually performed by semi-quantitative methylation-specific PCR, pyrosequencing, methylation-specific multiplexed ligation probe amplification (MS-MLPA)or methylation profiling arrays. MGMT immunohistochemistry is not recommended.<ref>{{Cite journal  | last1 = Quillien | first1 = V. | last2 = Lavenu | first2 = A. | last3 = Ducray | first3 = F. | last4 = Joly | first4 = MO. | last5 = Chinot | first5 = O. | last6 = Fina | first6 = F. | last7 = Sanson | first7 = M. | last8 = Carpentier | first8 = C. | last9 = Karayan-Tapon | first9 = L. | title = Validation of the high-performance of pyrosequencing for clinical MGMT testing on a cohort of glioblastoma patients from a prospective dedicated multicentric trial. | journal = Oncotarget | volume = 7 | issue = 38 | pages = 61916-61929 | month = 09 | year = 2016 | doi = 10.18632/oncotarget.11322 | PMID = 27542245 }}</ref><ref>{{Cite journal  | last1 = Trabelsi | first1 = S. | last2 = Mama | first2 = N. | last3 = Ladib | first3 = M. | last4 = Karmeni | first4 = N. | last5 = Haddaji Mastouri | first5 = M. | last6 = Chourabi | first6 = M. | last7 = Mokni | first7 = M. | last8 = Tlili | first8 = K. | last9 = Krifa | first9 = H. | title = MGMT methylation assessment in glioblastoma: MS-MLPA versus human methylation 450K beadchip array and immunohistochemistry. | journal = Clin Transl Oncol | volume = 18 | issue = 4 | pages = 391-7 | month = Apr | year = 2016 | doi = 10.1007/s12094-015-1381-0 | PMID = 26289551 }}</ref>

MGMT testing in neuropathology is usually performed by semi-quantitative methylation-specific PCR, pyrosequencing, methylation-specific multiplexed ligation probe amplification (MS-MLPA)or methylation profiling arrays. MGMT immunohistochemistry is not recommended.<ref>{{Cite journal  | last1 = Quillien | first1 = V. | last2 = Lavenu | first2 = A. | last3 = Ducray | first3 = F. | last4 = Joly | first4 = MO. | last5 = Chinot | first5 = O. | last6 = Fina | first6 = F. | last7 = Sanson | first7 = M. | last8 = Carpentier | first8 = C. | last9 = Karayan-Tapon | first9 = L. | title = Validation of the high-performance of pyrosequencing for clinical MGMT testing on a cohort of glioblastoma patients from a prospective dedicated multicentric trial. | journal = Oncotarget | volume = 7 | issue = 38 | pages = 61916-61929 | month = 09 | year = 2016 | doi = 10.18632/oncotarget.11322 | PMID = 27542245 }}</ref><ref>{{Cite journal  | last1 = Trabelsi | first1 = S. | last2 = Mama | first2 = N. | last3 = Ladib | first3 = M. | last4 = Karmeni | first4 = N. | last5 = Haddaji Mastouri | first5 = M. | last6 = Chourabi | first6 = M. | last7 = Mokni | first7 = M. | last8 = Tlili | first8 = K. | last9 = Krifa | first9 = H. | title = MGMT methylation assessment in glioblastoma: MS-MLPA versus human methylation 450K beadchip array and immunohistochemistry. | journal = Clin Transl Oncol | volume = 18 | issue = 4 | pages = 391-7 | month = Apr | year = 2016 | doi = 10.1007/s12094-015-1381-0 | PMID = 26289551 }}</ref>

[[Category:Neuropathology]]

[[Category:Neuropathology]]