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Difference between revisions of "Stains"
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→Immunohistochemistry: update
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==Immunohistochemistry== | ==Immunohistochemistry== | ||
{{main|Immunohistochemistry}} | {{main|Immunohistochemistry}} | ||
Abbreviated ''IHC''. | ===General=== | ||
*Abbreviated ''IHC''. | |||
Interpretation | ===Interpretation=== | ||
*Positive is ''brown''. | Simple version: | ||
*Negative tissue is ''light blue''. | *Positive is (usually): ''brown''. | ||
*Negative tissue is: ''light blue''. | |||
Important notes: | |||
*One has to know where the target is supposed to be, i.e. cytoplasm vs. cell membrane. | *One has to know where the target (of the antibody) is supposed to be, i.e. cytoplasm vs. cell membrane. | ||
*The edge of the tissue may have light staining - ''edge effect''. | *The edge of the tissue may have light staining - ''edge effect''. | ||
*If everything is brown... suspect that it didn't work. | *If everything is brown... suspect that it didn't work. | ||