Cytogenetics
This article deals with cytogenetics.
General
- Large changes (chromosomal).
- Maximum resolution 3-4 megabase pairs (3-4 million base pairs); may be less - dependent on band density.[1]
- Morphologic data.
Techniques
- ISH = in situ hybridization.
- FISH = fluorescent in situ hybridization.
- SISH = silver in situ hybridization.[2]
- Karyotyping.
Karyotyping
What?
- Metaphase nuclei.
How?
- Chromosomes are identified by:
- Size (chromosome 1 = largest, chromosome 22 = smallest).
- Position of centromere.
- Banding pattern - using (special) stains:
- Several different techniques (stains) are used:[3]
- Examples: Q-banding (Q=Quinacrine), C-banding (C=constitutive heterchromatin), G-banding (G=Giemsa), R-banding (R=reverse).
- Q-banding is the standard at UHN.
- Examples: Q-banding (Q=Quinacrine), C-banding (C=constitutive heterchromatin), G-banding (G=Giemsa), R-banding (R=reverse).
- Several different techniques (stains) are used:[3]
Analysis:
- Based on comparison to maps of the chromosomes (ideograms).
- Detailed ideograms are in ISCN 2009.[4]
- The detail of an ideogram is given in "band levels"; a 850 band ideogram has a higher resolution than a 400 band ideogram. The typical range for band level is 300-850. The band level, for a given specimen, is determined by an empirical standard and based on the number of bands an observer sees in a subset of the chromosomes.[5]
- Detailed ideograms are in ISCN 2009.[4]
Image:
Notes:
- Quinacrine dye - AT-rich regions brighter than GC-rich regions.
ISH
- Usu. interphase nuclei.
May be prepared from:
- In situ paraffin sections (section 4 micrometers thick).
- Isolated nuclei (section 10 micrometers thick).
- Cytospin.
- Cultured cells.
Probe types
Types:
- Fusion (two colours).
- Breakapart (two colours).
- Deletion/duplication (e.g. trisomy).
Image:
See also
References
- ↑ Humphrey, Peter A; Dehner, Louis P; Pfeifer, John D (2008). The Washington Manual of Surgical Pathology (1st ed.). Lippincott Williams & Wilkins. pp. 695. ISBN 978-0781765275.
- ↑ URL: http://www.immunoportal.com/modules.php?name=News&file=article&sid=186. Accessed on: 2 May 2011.
- ↑ URL: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC355922/. Accessed on: 10 May 2011.
- ↑ Shaffer, Lisa G.; Slovak, Marilyn L.; Campbell, Lynda J. (2009). ISCN 2009: an international system for human cytogenetic nomenclature (2009 ed.). S. Karger AG. ISBN 978-3805589857.
- ↑ Welborn, JL.; Welborn, R. (Dec 1993). "Banding resolution of human chromosomes: a method of accuracy and simplicity.". Am J Med Genet 47 (8): 1180-3. doi:10.1002/ajmg.1320470810. PMID 8291552.
External links
- Cytogenetics (homepage.mac.com) - has ideograms (banding maps) of the human chromosomes.