Tissue fixation

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Tissue fixation, usually just fixation, is an important part of tissue preparation for histologic examination. It is typically done with formalin.

Implications

Pathologist have a great lifestyle 'cause tissue takes long to fix; the penetration of tissue by formalin is 1 mm/hour.[1]

Ratio

The dictum is:[2]

  • The volume of fixative should be 10x the volume of specimen.

Tissue fixation

A list of fixatives:[3]

Fixative Comment
Formalin, neutral buffered standard fixative
Formalin, unbuffered ???
Glutaraldehyde electron microscopy ???
Ethanol cytopathology ???
Carnoy ???
Bouin toxic ???

Tissue fixation

Formalin

  • May be written (incorrectly) as "formulin".
  • Formaldehyde + methanol.

Fixing marking dye

To fix marking dye:

  • Formal-acetic alcohol (FAA):[4]
  • Bouin's solution.

Formal-acetic-alcohol

General:

  • Different recipes exist.

One recipe:[5]

  • Ethanol.
  • Acetic acid.
  • Formaldehyde.
  • Methanol.

See also

References

External links