Difference between revisions of "Papanicolaou stain"
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{{ Infobox stain | |||
| Name = {{PAGENAME}} | |||
| Image = Low grade squamous intraepithelial lesion.jpg | |||
| Width = | |||
| Caption = Low grade squamous intraepithelial lesion. | |||
| Abbrev = Pap stain | |||
| Synonyms = | |||
| Variants = | |||
| Similar = [[Romanowsky stain]]s | |||
| Use = the standard stain in [[cytopathology]] | |||
| Subspecial = | |||
| Interpret = blue/purple = nucleus, pink/green = cytoplasm, orange = keratin | |||
| Positive = | |||
| Negative = | |||
| Other = | |||
}} | |||
'''Papanicolaou stain''', abbreviated '''Pap stain''', is a standard [[stain]] used in [[cytopathology]]. It is a modified [[H&E stain]]. | '''Papanicolaou stain''', abbreviated '''Pap stain''', is a standard [[stain]] used in [[cytopathology]]. It is a modified [[H&E stain]]. | ||
Revision as of 04:10, 24 April 2016
Papanicolaou stain | |
---|---|
Stain in short | |
Low grade squamous intraepithelial lesion. | |
Abbreviation | Pap stain |
Similar stains | Romanowsky stains |
Use | the standard stain in cytopathology |
Interpretation | blue/purple = nucleus, pink/green = cytoplasm, orange = keratin |
Papanicolaou stain, abbreviated Pap stain, is a standard stain used in cytopathology. It is a modified H&E stain.
General
- Can be thought of as the H&E of cytopathology.
- It is a modified H&E stain.
- Specimens are fixed in ethanol.
- Good for seeing nuclear detail.
- Out-of-focus cytoplasm is translucent; allows one to focus overlapped cells in different planes.
Use
- Cytopathology.
Interpretation
- Blue/purple = nucleus.
- Green/pink = cytoplasm.
- Orange = keratin.
Images
Urine cytology - Pap stain. (WC)