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Difference between revisions of "Light microscopy"
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→Resolution: tweak
(create) |
(→Resolution: tweak) |
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<br> | <br> | ||
Where: | Where: | ||
*R = resolving distance; smaller better. | |||
*NA = 0.25 - 1.4, >1.0 is oil immersion. | *NA = 0.25 - 1.4, >1.0 is oil immersion. | ||
*gamma = wave length of light. | *gamma = wave length of light. | ||
Closure of the condenser diaphragm results in a loss of resolution, i.e. R is larger. | Closure of the condenser diaphragm results in a loss of resolution, i.e. R is larger.<br> | ||
R = 1.22 * {gamma \over ( D/2*f )} | <math>R = 1.22 * {gamma \over ( D/2*f )}</math> | ||
Notes: | Notes: | ||