Difference between revisions of "Tissue fixation"
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*[http://www.wisegeek.com/what-is-formalin.htm What is formalin (wisegeek.com)]. | *[http://www.wisegeek.com/what-is-formalin.htm What is formalin (wisegeek.com)]. | ||
*[http://en.wikipedia.org/wiki/Fixation_%28histology%29 Fixation (WP)]. | *[http://en.wikipedia.org/wiki/Fixation_%28histology%29 Fixation (WP)]. | ||
*[http://www-bioc.rice.edu/bios576/histology/histology.htm General overview of histology (rice.edu)]. | |||
[[Category:Basics]] | [[Category:Basics]] | ||
Revision as of 20:33, 11 September 2011
Tissue fixation, usually just fixation, is an important part of tissue preparation for histologic examination. It is typically done with formalin.
Implications
Pathologist have a great lifestyle 'cause tissue takes long to fix; the penetration of tissue by formalin is 1 mm/hour.[1]
Fixing marking dye
To fix marking dye:
- Formal-acetic alcohol (FAA):[2]
- Bouin's solution.
Tissue fixation
A list of fixatives:[3]
| Fixative | Comment |
|---|---|
| Formalin, neutral buffered | standard fixative |
| Formalin, unbuffered | ??? |
| Glutaraldehyde | electron microscopy ??? |
| Ethanol | cytopathology ??? |
| Carnoy | ??? |
| Bouin | toxic ??? |
Tissue fixation
Formalin
- May be written (incorrectly) as "formulin".
- Formaldehyde + methanol.
Dye fixation
Formal-acetic-alcohol
General:
- Different recipes exist.
One recipe:[4]
- Ethanol.
- Acetic acid.
- Formaldehyde.
- Methanol.
See also
References
- ↑ Gross rounds. 14 August 2009.
- ↑ URL: http://www.brunelmicroscopes.co.uk/acatalog/Solvents___Reagents.html. Accessed on: 5 January 2011.
- ↑ Hunt, JL. (Feb 2008). "Molecular pathology in anatomic pathology practice: a review of basic principles.". Arch Pathol Lab Med 132 (2): 248-60. doi:10.1043/1543-2165(2008)132[248:MPIAPP]2.0.CO;2. PMID 18251585. http://www.archivesofpathology.org/doi/pdf/10.1043/1543-2165%282008%29132%5B248%3AMPIAPP%5D2.0.CO%3B2.
- ↑ URL: https://fscimage.fishersci.com/msds/45357.htm. Accessed on: 5 January 2011.