Difference between revisions of "Paired box gene 8"
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m (PAX8 polyclonal antibodies can cross react with PAX5 = false positive) |
(→Negative staining: +mesothelioma) |
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**Can help differentiate from lung NETs. | **Can help differentiate from lung NETs. | ||
*Epithelial component of thymic tumors 77-100% depending on sub-type<ref name=pmid21552115/><ref name=pmid21836478>{{cite journal |vauthors=Weissferdt A, Moran CA |title=Pax8 expression in thymic epithelial neoplasms: an immunohistochemical analysis |journal=Am. J. Surg. Pathol. |volume=35 |issue=9 |pages=1305–10 |date=September 2011 |pmid=21836478 |doi=10.1097/PAS.0b013e3182260735 |url=}}</ref> | *Epithelial component of thymic tumors 77-100% depending on sub-type<ref name=pmid21552115/><ref name=pmid21836478>{{cite journal |vauthors=Weissferdt A, Moran CA |title=Pax8 expression in thymic epithelial neoplasms: an immunohistochemical analysis |journal=Am. J. Surg. Pathol. |volume=35 |issue=9 |pages=1305–10 |date=September 2011 |pmid=21836478 |doi=10.1097/PAS.0b013e3182260735 |url=}}</ref> | ||
===May be positive=== | |||
*Peritoneal [[malignant mesothelioma]] (5 +ve of 27<ref name=pmid28877056>{{cite journal |vauthors=Chapel DB, Husain AN, Krausz T, McGregor SM |title=PAX8 Expression in a Subset of Malignant Peritoneal Mesotheliomas and Benign Mesothelium has Diagnostic Implications in the Differential Diagnosis of Ovarian Serous Carcinoma |journal=Am. J. Surg. Pathol. |volume=41 |issue=12 |pages=1675–1682 |date=December 2017 |pmid=28877056 |doi=10.1097/PAS.0000000000000935 |url=}}</ref>). | |||
==Negative staining== | ==Negative staining== | ||
Revision as of 15:30, 3 October 2020
| Paired box gene 8 | |
|---|---|
| Immunostain in short | |
 PAX8 staining in a poorly differentiated carcinoma. | |
| Abbreviation | PAX8 |
| Use | RCC versus other, gynecologic tract malignancy versus other |
| Subspeciality | gynecologic pathology, genitourinary pathology |
| Normal staining pattern | nuclear stain |
| Positive | renal cell carcinomas, renal oncocytoma, thyroid tumours, serous carcinoma, endometrial carcinomas |
Paired box gene 8, commonly known by the abbreviation PAX8, is a commonly used immunostain in gynecologic pathology and genitourinary pathology.
Tumours with positive staining
- Thyroid tumours ~ 90%.[1]
- Renal cell carcinomas ~ 90%.[1]
- Renal oncocytomas ~ 80%.[1]
- High-grade ovarian serous carcinomas ~ 99%.[1]
- Nonserous epithelial ovarian neoplasms ~ 70%.[1]
- Endometrial carcinomas ~ 98%.[1]
- Pancreatic neuroendocrine tumour ~ 74%.[2]
- Can help differentiate from lung NETs.
- Epithelial component of thymic tumors 77-100% depending on sub-type[1][3]
May be positive
- Peritoneal malignant mesothelioma (5 +ve of 27[4]).
Negative staining
- Urothelial carcinoma - can be positive (3 positive/18 bladder tumours[1]).
- Lymphoma (beware false positive staining due to polyclonal PAX8 antibodies cross-reacting with PAX5)[5]
- Malignant melanoma.[6]
See also
References
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 Laury, AR.; Perets, R.; Piao, H.; Krane, JF.; Barletta, JA.; French, C.; Chirieac, LR.; Lis, R. et al. (Jun 2011). "A comprehensive analysis of PAX8 expression in human epithelial tumors.". Am J Surg Pathol 35 (6): 816-26. doi:10.1097/PAS.0b013e318216c112. PMID 21552115.
- ↑ Sangoi, AR.; Ohgami, RS.; Pai, RK.; Beck, AH.; McKenney, JK.; Pai, RK. (Mar 2011). "PAX8 expression reliably distinguishes pancreatic well-differentiated neuroendocrine tumors from ileal and pulmonary well-differentiated neuroendocrine tumors and pancreatic acinar cell carcinoma.". Mod Pathol 24 (3): 412-24. doi:10.1038/modpathol.2010.176. PMID 20890270.
- ↑ "Pax8 expression in thymic epithelial neoplasms: an immunohistochemical analysis". Am. J. Surg. Pathol. 35 (9): 1305–10. September 2011. doi:10.1097/PAS.0b013e3182260735. PMID 21836478.
- ↑ "PAX8 Expression in a Subset of Malignant Peritoneal Mesotheliomas and Benign Mesothelium has Diagnostic Implications in the Differential Diagnosis of Ovarian Serous Carcinoma". Am. J. Surg. Pathol. 41 (12): 1675–1682. December 2017. doi:10.1097/PAS.0000000000000935. PMID 28877056.
- ↑ "N-terminal PAX8 polyclonal antibody shows cross-reactivity with N-terminal region of PAX5 and is responsible for reports of PAX8 positivity in malignant lymphomas". Mod. Pathol. 25 (2): 231–6. February 2012. doi:10.1038/modpathol.2011.162. PMID 22037256.
- ↑ Tacha, D.; Zhou, D.; Cheng, L. (Jul 2011). "Expression of PAX8 in normal and neoplastic tissues: a comprehensive immunohistochemical study.". Appl Immunohistochem Mol Morphol 19 (4): 293-9. doi:10.1097/PAI.0b013e3182025f66. PMID 21285870.