Difference between revisions of "Molecular pathology"
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==Molecular== | |||
General: | General: | ||
*Very small changes. | *Very small changes. | ||
| Line 18: | Line 18: | ||
*DNA sequencing - RT-PCR. | *DNA sequencing - RT-PCR. | ||
*RNA sequencing. | *RNA sequencing. | ||
*Southern blot. | |||
===Cytogenetics | ===Tests=== | ||
*Hereditary: | |||
**Factor V. | |||
**Factor II. | |||
**[[Hemochromatosis]]. | |||
**Malignant hyperthermia. | |||
**Hereditary [[amyloidosis]]. | |||
**Alpha thalassemia. | |||
*[[Lymphoma]]. | |||
**Follicular lymphoma vs. follicular hyperplasia (BCL2). | |||
**T cell neoplasia. | |||
**B cell neoplasia. | |||
**PTLPD vs. rejection. | |||
**HHV associated lymphomas. | |||
*Leukemia. | |||
*Carcinoma: | |||
**Nasopharyngeal (EBV quantitation). | |||
**HPV testing. | |||
**Metastatic [[colorectal carinoma]]. | |||
**Non small cell [[lung cancer]]. | |||
**Papillary thyroid carcinoma. | |||
*Other: | |||
**Oligodendroglioma. | |||
**Identity testing (15 STRs and amelogenin (XY) loci). | |||
**Melanoma (KIT, BRAF). | |||
**[[Synovial sarcoma]]. | |||
**Myeloproliferative disorders. | |||
**AML, mastocytosis, GIST. | |||
==Cytogenetics== | |||
General: | General: | ||
*Large changes (chromosomal). | *Large changes (chromosomal). | ||
Revision as of 12:51, 3 May 2011
Molecular pathology is the future of pathology.
Overview
Molecular pathology can be divided as follows:
| Molecular pathology | |||||||||||||||||||
| Molecular | Cytogenetics | ||||||||||||||||||
Molecular
General:
- Very small changes.
Techniques:
- DNA sequencing - RT-PCR.
- RNA sequencing.
- Southern blot.
Tests
- Hereditary:
- Factor V.
- Factor II.
- Hemochromatosis.
- Malignant hyperthermia.
- Hereditary amyloidosis.
- Alpha thalassemia.
- Lymphoma.
- Follicular lymphoma vs. follicular hyperplasia (BCL2).
- T cell neoplasia.
- B cell neoplasia.
- PTLPD vs. rejection.
- HHV associated lymphomas.
- Leukemia.
- Carcinoma:
- Nasopharyngeal (EBV quantitation).
- HPV testing.
- Metastatic colorectal carinoma.
- Non small cell lung cancer.
- Papillary thyroid carcinoma.
- Other:
- Oligodendroglioma.
- Identity testing (15 STRs and amelogenin (XY) loci).
- Melanoma (KIT, BRAF).
- Synovial sarcoma.
- Myeloproliferative disorders.
- AML, mastocytosis, GIST.
Cytogenetics
General:
- Large changes (chromosomal).
- Maximum resolution 3-4 megabase pairs (3-4 million base pairs); may be less - dependent on band density.[1]
Techniques:
- ISH = in situ hybridization.
- FISH = fluorescent in situ hybridization.
- SISH = silver in situ hybridization.[2]
Image:
World protein databank
I can't help think it is ironic that the protein databank goal is to maintain a free and publicly available archive,[3] yet the announcement is in pay-for-access journal (Nature Structual Biology).[4]
Wnt/beta-catenin pathway
Important in hepatoblastomas.[5]
See also
References
- ↑ Humphrey, Peter A; Dehner, Louis P; Pfeifer, John D (2008). The Washington Manual of Surgical Pathology (1st ed.). Lippincott Williams & Wilkins. pp. 695. ISBN 978-0781765275.
- ↑ URL: http://www.immunoportal.com/modules.php?name=News&file=article&sid=186. Accessed on: 2 May 2011.
- ↑ Worldwide Protein Data Bank. URL: http://www.wwpdb.org/faq.html Accessed on: April 22, 2009.
- ↑ Berman H, Henrick K, Nakamura H (December 2003). "Announcing the worldwide Protein Data Bank". Nat. Struct. Biol. 10 (12): 980. doi:10.1038/nsb1203-980. PMID 14634627.
- ↑ Cotran, Ramzi S.; Kumar, Vinay; Fausto, Nelson; Nelso Fausto; Robbins, Stanley L.; Abbas, Abul K. (2005). Robbins and Cotran pathologic basis of disease (7th ed.). St. Louis, Mo: Elsevier Saunders. pp. 923. ISBN 0-7216-0187-1.