Difference between revisions of "Papanicolaou stain"
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Image: | Image:Low grade squamous intraepithelial lesion.jpg|LSIL - Pap stain. (WC) | ||
Image:High-grade squamous intraepithelial lesion.jpg|HSIL - Pap stain. (WC) | |||
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Image:Urine_citology_urothelial_carcinoma_2.jpg | [[Urine cytology]] - Pap stain. (WC) | |||
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Revision as of 04:08, 24 April 2016
Papanicolaou stain, abbreviated Pap stain, is a standard stain used in cytopathology. It is a modified H&E stain.
General
- Can be thought of as the H&E of cytopathology.
- It is a modified H&E stain.
- Specimens are fixed in ethanol.
- Good for seeing nuclear detail.
- Out-of-focus cytoplasm is translucent; allows one to focus overlapped cells in different planes.
Use
- Cytopathology.
Interpretation
- Blue/purple = nucleus.
- Green/pink = cytoplasm.
- Orange = keratin.
Images
Urine cytology - Pap stain. (WC)