Difference between revisions of "Papanicolaou stain"
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'''Papanicolaou stain''', abbreviated '''Pap stain''', is a standard [[stain]] used in [[cytopathology]]. It is a modified [[H&E stain]]. | |||
==General== | |||
*Can be thought of as the [[H&E stain|H&E]] of [[cytopathology]]. | |||
**It is a modified [[H&E stain]]. | |||
*Specimens are fixed in ethanol. | |||
*Good for seeing nuclear detail. | |||
*Out-of-focus cytoplasm is translucent; allows one to focus overlapped cells in different planes. | |||
==Use== | |||
*Cytopathology. | |||
==Interpretation== | |||
*Blue/purple = nucleus. | |||
*Green/pink = cytoplasm. | |||
*Orange = keratin. | |||
===Image=== | |||
<gallery> | |||
Image:Urine_citology_urothelial_carcinoma_2.jpg | Pap stain - [[urine cytology]] (WC) | |||
</gallery> | |||
==See also== | |||
*[[Stains]]. | |||
[[Category:Stains]] | [[Category:Stains]] |
Revision as of 04:03, 24 April 2016
Papanicolaou stain, abbreviated Pap stain, is a standard stain used in cytopathology. It is a modified H&E stain.
General
- Can be thought of as the H&E of cytopathology.
- It is a modified H&E stain.
- Specimens are fixed in ethanol.
- Good for seeing nuclear detail.
- Out-of-focus cytoplasm is translucent; allows one to focus overlapped cells in different planes.
Use
- Cytopathology.
Interpretation
- Blue/purple = nucleus.
- Green/pink = cytoplasm.
- Orange = keratin.
Image
Pap stain - urine cytology (WC)