Difference between revisions of "Light microscopy"

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===Numerical aperture===
===Numerical aperture===
NA = numerical aperature.
NA = numerical aperture.


General formula for NA:<ref>URL: [http://en.wikipedia.org/wiki/Numerical_aperture http://en.wikipedia.org/wiki/Numerical_aperture]. Accessed on: 21 January 2011.</ref><br>
General formula for NA:<ref>URL: [http://en.wikipedia.org/wiki/Numerical_aperture http://en.wikipedia.org/wiki/Numerical_aperture]. Accessed on: 21 January 2011.</ref><br>
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#Any specimen on stage.
#Any specimen on stage.
#Focus.
#Focus.
#Adj. field aperature (bottom) - to obscure periphery of field of view (FOV).
#Adjust field aperture (bottom) - to obscure periphery of field of view (FOV).
#Raise or lower condenser until field aperature diaphragm clearly focused.
#Raise or lower condenser until field aperture diaphragm clearly focused.
#+/-Center 'field aperature diaphragm - using condenser centering screws.
#+/-Center 'field aperture diaphragm - using condenser centering screws.


==See also==
==See also==