Light microscopy: Difference between revisions
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This article examines '''light microscopy'''. | [[Image:Optical microscope nikon alphaphot +.jpg|thumb|250px|A light microscope.]] | ||
This article examines '''light microscopy''', abbreviated '''LM'''. | |||
==Resolution== | ==Resolution== | ||
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*e = resolution. | *e = resolution. | ||
== | ===Increasing the DOF=== | ||
*DOF can be increased by ''focus stacking''. | |||
Software: | |||
*[http://hugin.sourceforge.net/ Hugin (sourceforge.net)] - does ''focus stacking'' and ''stitching''. | |||
Image: | |||
*[http://commons.wikimedia.org/wiki/File:Focus_stacking_Tachinid_fly.jpg Example of focus stacking (WC)]. | |||
==Köhler illumination== | |||
===Rationale=== | ===Rationale=== | ||
* | *Even light distribution (avoid [[vignetting]]). | ||
===Procedure=== | ===Procedure=== | ||
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*[[Microphotography]]. | *[[Microphotography]]. | ||
*[[Electron microscopy]]. | *[[Electron microscopy]]. | ||
*[[Polarization of light]]. | |||
==References== | ==References== | ||