Difference between revisions of "Carbonic anhydrase 9"
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'''Carbonic anhydrase 9''', abbreviated '''CA9''', is a common [[immunostain]] used in [[genitourinary pathology]]. | '''Carbonic anhydrase 9''', abbreviated '''CA9''' and '''CAIX''', is a common [[immunostain]] used in [[genitourinary pathology]]. | ||
==General== | ==General== | ||
*Several clones available - | *Several clones available - clone M75 most common.<ref name=pmid18300814>{{Cite journal | last1 = Al-Ahmadie | first1 = HA. | last2 = Alden | first2 = D. | last3 = Qin | first3 = LX. | last4 = Olgac | first4 = S. | last5 = Fine | first5 = SW. | last6 = Gopalan | first6 = A. | last7 = Russo | first7 = P. | last8 = Motzer | first8 = RJ. | last9 = Reuter | first9 = VE. | title = Carbonic anhydrase IX expression in clear cell renal cell carcinoma: an immunohistochemical study comparing 2 antibodies. | journal = Am J Surg Pathol | volume = 32 | issue = 3 | pages = 377-82 | month = Mar | year = 2008 | doi = 10.1097/PAS.0b013e3181570343 | PMID = 18300814 }}</ref> | ||
==Staining== | ==Staining== | ||
===Positive=== | ===Positive=== | ||
*[[Clear cell renal cell carcinoma]].<ref name=pmid21088149>{{Cite journal | last1 = Genega | first1 = EM. | last2 = Ghebremichael | first2 = M. | last3 = Najarian | first3 = R. | last4 = Fu | first4 = Y. | last5 = Wang | first5 = Y. | last6 = Argani | first6 = P. | last7 = Grisanzio | first7 = C. | last8 = Signoretti | first8 = S. | title = Carbonic anhydrase IX expression in renal neoplasms: correlation with tumor type and grade. | journal = Am J Clin Pathol | volume = 134 | issue = 6 | pages = 873-9 | month = Dec | year = 2010 | doi = 10.1309/AJCPPPR57HNJMSLZ | PMID = 21088149 }}</ref> | *[[Clear cell renal cell carcinoma]].<ref name=pmid21088149>{{Cite journal | last1 = Genega | first1 = EM. | last2 = Ghebremichael | first2 = M. | last3 = Najarian | first3 = R. | last4 = Fu | first4 = Y. | last5 = Wang | first5 = Y. | last6 = Argani | first6 = P. | last7 = Grisanzio | first7 = C. | last8 = Signoretti | first8 = S. | title = Carbonic anhydrase IX expression in renal neoplasms: correlation with tumor type and grade. | journal = Am J Clin Pathol | volume = 134 | issue = 6 | pages = 873-9 | month = Dec | year = 2010 | doi = 10.1309/AJCPPPR57HNJMSLZ | PMID = 21088149 }}</ref> | ||
**95% +ve with clone NB100-417 and clone M75.<ref name=pmid18300814>{{Cite journal | last1 = Al-Ahmadie | first1 = HA. | last2 = Alden | first2 = D. | last3 = Qin | first3 = LX. | last4 = Olgac | first4 = S. | last5 = Fine | first5 = SW. | last6 = Gopalan | first6 = A. | last7 = Russo | first7 = P. | last8 = Motzer | first8 = RJ. | last9 = Reuter | first9 = VE. | title = Carbonic anhydrase IX expression in clear cell renal cell carcinoma: an immunohistochemical study comparing 2 antibodies. | journal = Am J Surg Pathol | volume = 32 | issue = 3 | pages = 377-82 | month = Mar | year = 2008 | doi = 10.1097/PAS.0b013e3181570343 | PMID = 18300814 }}</ref> | |||
*[[Malignant mesothelioma|Epithelioid mesothelioma]].<ref name=pmid23503645>{{Cite journal | last1 = Ordóñez | first1 = NG. | title = Value of PAX8, PAX2, napsin A, carbonic anhydrase IX, and claudin-4 immunostaining in distinguishing pleural epithelioid mesothelioma from metastatic renal cell carcinoma. | journal = Mod Pathol | volume = 26 | issue = 8 | pages = 1132-43 | month = Aug | year = 2013 | doi = 10.1038/modpathol.2013.34 | PMID = 23503645 }}</ref> | |||
*Benign mesothelium.<ref name=pmid24926090>{{Cite journal | last1 = Ananthanarayanan | first1 = V. | last2 = Tretiakova | first2 = M. | last3 = Husain | first3 = AN. | last4 = Krausz | first4 = T. | last5 = Antic | first5 = T. | title = Carbonic anhydrase IX (CAIX) does not differentiate between benign and malignant mesothelium. | journal = Am J Clin Pathol | volume = 142 | issue = 1 | pages = 82-7 | month = Jul | year = 2014 | doi = 10.1309/AJCP8BJ8CJXUEHZD | PMID = 24926090 }}</ref> | |||
===Negative=== | ===Negative=== | ||
*[[Xp11.2 translocation carcinoma]].<ref name=pmid21088149/> | *[[Xp11.2 translocation carcinoma]].<ref name=pmid21088149/> | ||
*Breast carcinoma.<ref name=pmid24436269>{{Cite journal | last1 = Luong-Player | first1 = A. | last2 = Liu | first2 = H. | last3 = Wang | first3 = HL. | last4 = Lin | first4 = F. | title = Immunohistochemical reevaluation of carbonic anhydrase IX (CA IX) expression in tumors and normal tissues. | journal = Am J Clin Pathol | volume = 141 | issue = 2 | pages = 219-25 | month = Feb | year = 2014 | doi = 10.1309/AJCPVJDS28KNYZLD | PMID = 24436269 }}</ref> ‡ | |||
*[[Seminoma]].<ref name=pmid24436269/> | |||
Note: | |||
*‡ A paper by Furjelová ''et al.'' found different results.<ref name=pmid23816158>{{Cite journal | last1 = Furjelová | first1 = M. | last2 = Kovalská | first2 = M. | last3 = Jurková | first3 = K. | last4 = Horáček | first4 = J. | last5 = Carbolová | first5 = T. | last6 = Adamkov | first6 = M. | title = Carbonic anhydrase IX: a promising diagnostic and prognostic biomarker in breast carcinoma. | journal = Acta Histochem | volume = 116 | issue = 1 | pages = 89-93 | month = Jan | year = 2014 | doi = 10.1016/j.acthis.2013.05.009 | PMID = 23816158 }}</ref> | |||
===Images=== | |||
<gallery> | |||
Image: Carbonic anhydrase 9 -- intermed mag.jpg | CA9 - intermed. mag. (WC) | |||
Image: Carbonic anhydrase 9 - alt -- high mag.jpg | CA9 - high mag. (WC) | |||
Image: Carbonic anhydrase 9 -- very high mag.jpg | CA9 - very high mag. (WC) | |||
</gallery> | |||
==See also== | ==See also== | ||
*[[Immunohistochemistry]]. | *[[Immunohistochemistry]]. | ||
*[[RCC (immunostain)]]. | |||
==References== | ==References== | ||
Latest revision as of 04:56, 3 March 2019
Carbonic anhydrase 9, abbreviated CA9 and CAIX, is a common immunostain used in genitourinary pathology.
General
- Several clones available - clone M75 most common.[1]
Staining
Positive
- Clear cell renal cell carcinoma.[2]
- 95% +ve with clone NB100-417 and clone M75.[1]
- Epithelioid mesothelioma.[3]
- Benign mesothelium.[4]
Negative
- Xp11.2 translocation carcinoma.[2]
- Breast carcinoma.[5] ‡
- Seminoma.[5]
Note:
- ‡ A paper by Furjelová et al. found different results.[6]
Images
CA9 - intermed. mag. (WC)
CA9 - high mag. (WC)
CA9 - very high mag. (WC)
See also
References
- ↑ 1.0 1.1 Al-Ahmadie, HA.; Alden, D.; Qin, LX.; Olgac, S.; Fine, SW.; Gopalan, A.; Russo, P.; Motzer, RJ. et al. (Mar 2008). "Carbonic anhydrase IX expression in clear cell renal cell carcinoma: an immunohistochemical study comparing 2 antibodies.". Am J Surg Pathol 32 (3): 377-82. doi:10.1097/PAS.0b013e3181570343. PMID 18300814.
- ↑ 2.0 2.1 Genega, EM.; Ghebremichael, M.; Najarian, R.; Fu, Y.; Wang, Y.; Argani, P.; Grisanzio, C.; Signoretti, S. (Dec 2010). "Carbonic anhydrase IX expression in renal neoplasms: correlation with tumor type and grade.". Am J Clin Pathol 134 (6): 873-9. doi:10.1309/AJCPPPR57HNJMSLZ. PMID 21088149.
- ↑ Ordóñez, NG. (Aug 2013). "Value of PAX8, PAX2, napsin A, carbonic anhydrase IX, and claudin-4 immunostaining in distinguishing pleural epithelioid mesothelioma from metastatic renal cell carcinoma.". Mod Pathol 26 (8): 1132-43. doi:10.1038/modpathol.2013.34. PMID 23503645.
- ↑ Ananthanarayanan, V.; Tretiakova, M.; Husain, AN.; Krausz, T.; Antic, T. (Jul 2014). "Carbonic anhydrase IX (CAIX) does not differentiate between benign and malignant mesothelium.". Am J Clin Pathol 142 (1): 82-7. doi:10.1309/AJCP8BJ8CJXUEHZD. PMID 24926090.
- ↑ 5.0 5.1 Luong-Player, A.; Liu, H.; Wang, HL.; Lin, F. (Feb 2014). "Immunohistochemical reevaluation of carbonic anhydrase IX (CA IX) expression in tumors and normal tissues.". Am J Clin Pathol 141 (2): 219-25. doi:10.1309/AJCPVJDS28KNYZLD. PMID 24436269.
- ↑ Furjelová, M.; Kovalská, M.; Jurková, K.; Horáček, J.; Carbolová, T.; Adamkov, M. (Jan 2014). "Carbonic anhydrase IX: a promising diagnostic and prognostic biomarker in breast carcinoma.". Acta Histochem 116 (1): 89-93. doi:10.1016/j.acthis.2013.05.009. PMID 23816158.