Difference between revisions of "Fontana-Masson stain"
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[[Image:18S00822 axillary metastasis Masson Fontana x40.jpg|thumb|Masson-Fontana staining of metastatic melanoma.]] | |||
'''Fontana-Masson stain''' is a special stain that marks the pigment melanin. It is a type of silver stain.{{fact}} | '''Fontana-Masson stain''' is a special stain that marks the pigment melanin. It is a type of silver stain.{{fact}} | ||
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*Stain for melanin. | *Stain for melanin. | ||
**Displays melanin - whether it be in melanocytes, keratinocytes or melanophages. | **Displays melanin - whether it be in melanocytes, keratinocytes or melanophages. | ||
**IHC stains, i.e. ''Melan A, SOX10 or MITF'' are preferable for displaying ''melanocytes''. | **[[IHC stains]], i.e. ''Melan A, SOX10 or [[MITF]]'' are preferable for displaying ''melanocytes''. | ||
**Used to differentiate brown pigments (lipofuscin, hemosiderin, melanin).<ref>URL: [http://education.vetmed.vt.edu/curriculum/VM8054/labs/Lab2/Examples/exfontana.htm http://education.vetmed.vt.edu/curriculum/VM8054/labs/Lab2/Examples/exfontana.htm]. Accessed on: 5 May 2010.</ref> | **Used to differentiate brown pigments (lipofuscin, hemosiderin, melanin).<ref>URL: [http://education.vetmed.vt.edu/curriculum/VM8054/labs/Lab2/Examples/exfontana.htm http://education.vetmed.vt.edu/curriculum/VM8054/labs/Lab2/Examples/exfontana.htm]. Accessed on: 5 May 2010.</ref> | ||
**Used to document Minocycline type II drug induced pigment deposition | **Used to document Minocycline type II drug induced pigment deposition | ||
***Minocycline pigment, Type II will stain with both the Fontana-Masson stain AND the Perls iron stain.<ref name=pmid19595269>{{cite journal |author=Geria AN, Tajirian AL, Kihiczak G, Schwartz RA |title=Minocycline-induced skin pigmentation: an update |journal=Acta Dermatovenerol Croat |volume=17 |issue=2 |pages=123–6 |year=2009 |pmid=19595269 |doi= |url=}}</ref> | ***Minocycline pigment, Type II will stain with both the Fontana-Masson stain AND the [[Iron stain|Perls iron stain]].<ref name=pmid19595269>{{cite journal |author=Geria AN, Tajirian AL, Kihiczak G, Schwartz RA |title=Minocycline-induced skin pigmentation: an update |journal=Acta Dermatovenerol Croat |volume=17 |issue=2 |pages=123–6 |year=2009 |pmid=19595269 |doi= |url=}}</ref> | ||
**Used in the differential diagnosis of hypomelanosis | **Used in the differential diagnosis of hypomelanosis | ||
***Idiopathic hypomelanosis will demonstrate ONLY loss of melanin with the Fontana-Masson stain - melanocytes will not be absent with the Melan A stain. | ***Idiopathic hypomelanosis will demonstrate ONLY loss of melanin with the Fontana-Masson stain - melanocytes will not be absent with the Melan A stain. | ||
***Vitiligo will show loss of melanin AND loss of melanocytes with a Melan A stain. | ***Vitiligo will show loss of melanin AND loss of melanocytes with a Melan A stain. | ||
Image: | <!--Image: | ||
*[http://education.vetmed.vt.edu/curriculum/VM8054/labs/Lab2/Examples/exfontana.htm Fontana-Masson stain (vetmed.vt.edu)]. | *[http://education.vetmed.vt.edu/curriculum/VM8054/labs/Lab2/Examples/exfontana.htm Fontana-Masson stain (vetmed.vt.edu)]. | ||
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==See also== | ==See also== | ||
*[[Stains]]. | *[[Stains]]. | ||
*[[Minocycline associated thyroid pigmentation]]. | |||
==References== | ==References== | ||
Latest revision as of 15:28, 5 November 2021
Fontana-Masson stain is a special stain that marks the pigment melanin. It is a type of silver stain.[citation needed]
It is also known as Masson-Fontana stain,[1] Fontana-Masson stain for melanin, and melanin stain.
Stains
- Melanin.
- "Argentaffin granules" of the digestive tract.
- Pigment deposition due to minocycline treatment.[2]
Use
- Stain for melanin.
- Displays melanin - whether it be in melanocytes, keratinocytes or melanophages.
- IHC stains, i.e. Melan A, SOX10 or MITF are preferable for displaying melanocytes.
- Used to differentiate brown pigments (lipofuscin, hemosiderin, melanin).[3]
- Used to document Minocycline type II drug induced pigment deposition
- Minocycline pigment, Type II will stain with both the Fontana-Masson stain AND the Perls iron stain.[4]
- Used in the differential diagnosis of hypomelanosis
- Idiopathic hypomelanosis will demonstrate ONLY loss of melanin with the Fontana-Masson stain - melanocytes will not be absent with the Melan A stain.
- Vitiligo will show loss of melanin AND loss of melanocytes with a Melan A stain.
See also
References
- ↑ Gaitanis, G.; Chasapi, V.; Velegraki, A. (Aug 2005). "Novel application of the masson-fontana stain for demonstrating Malassezia species melanin-like pigment production in vitro and in clinical specimens.". J Clin Microbiol 43 (8): 4147-51. doi:10.1128/JCM.43.8.4147-4151.2005. PMID 16081962.
- ↑ Patterson, JW.; Wilson, B.; Wick, MR.; Heath, C. (Nov 2004). "Hyperpigmented scar due to minocycline therapy.". Cutis 74 (5): 293-8. PMID 15605966.
- ↑ URL: http://education.vetmed.vt.edu/curriculum/VM8054/labs/Lab2/Examples/exfontana.htm. Accessed on: 5 May 2010.
- ↑ Geria AN, Tajirian AL, Kihiczak G, Schwartz RA (2009). "Minocycline-induced skin pigmentation: an update". Acta Dermatovenerol Croat 17 (2): 123–6. PMID 19595269.