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Difference between revisions of "Fontana-Masson stain"
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# | '''Fontana-Masson stain''' is a special stain that marks the pigment melanin. | ||
It is also known as ''Masson-Fontana stain'',<ref name=pmid16081962>{{Cite journal | last1 = Gaitanis | first1 = G. | last2 = Chasapi | first2 = V. | last3 = Velegraki | first3 = A. | title = Novel application of the masson-fontana stain for demonstrating Malassezia species melanin-like pigment production in vitro and in clinical specimens. | journal = J Clin Microbiol | volume = 43 | issue = 8 | pages = 4147-51 | month = Aug | year = 2005 | doi = 10.1128/JCM.43.8.4147-4151.2005 | PMID = 16081962 }}</ref> ''Fontana-Masson stain for melanin'', ''melanin stain''. | |||
*A type of silver stain. | |||
==Stains== | |||
#Melanin. | |||
#"Argentaffin granules" of the digestive tract. | |||
#Pigment deposition due to minocycline treatment.<ref name=pmid15605966>{{Cite journal | last1 = Patterson | first1 = JW. | last2 = Wilson | first2 = B. | last3 = Wick | first3 = MR. | last4 = Heath | first4 = C. | title = Hyperpigmented scar due to minocycline therapy. | journal = Cutis | volume = 74 | issue = 5 | pages = 293-8 | month = Nov | year = 2004 | doi = | PMID = 15605966 }}</ref> | |||
==Use== | |||
*Stain for melanin. | |||
**Displays melanin - whether it be in melanocytes, keratinocytes or melanophages. | |||
**IHC stains, i.e. ''Melan A, SOX10 or MITF'' are preferable for displaying ''melanocytes''. | |||
**Used to differentiate brown pigments (lipofuscin, hemosiderin, melanin).<ref>URL: [http://education.vetmed.vt.edu/curriculum/VM8054/labs/Lab2/Examples/exfontana.htm http://education.vetmed.vt.edu/curriculum/VM8054/labs/Lab2/Examples/exfontana.htm]. Accessed on: 5 May 2010.</ref> | |||
**Used to document Minocycline type II drug induced pigment deposition | |||
***Minocycline pigment, Type II will stain with both the Fontana-Masson stain AND the Perls iron stain.<ref name=pmid19595269>{{cite journal |author=Geria AN, Tajirian AL, Kihiczak G, Schwartz RA |title=Minocycline-induced skin pigmentation: an update |journal=Acta Dermatovenerol Croat |volume=17 |issue=2 |pages=123–6 |year=2009 |pmid=19595269 |doi= |url=}}</ref> | |||
**Used in the differential diagnosis of hypomelanosis | |||
***Idiopathic hypomelanosis will demonstrate ONLY loss of melanin with the Fontana-Masson stain - melanocytes will not be absent with the Melan A stain. | |||
***Vitiligo will show loss of melanin AND loss of melanocytes with a Melan A stain. | |||
Image: | |||
*[http://education.vetmed.vt.edu/curriculum/VM8054/labs/Lab2/Examples/exfontana.htm Fontana-Masson stain (vetmed.vt.edu)]. | |||
==See also== | |||
*[[Stains]]. | |||
==References== | |||
{{Reflist|1}} | |||
[[Category:Stains]] | [[Category:Stains]] | ||