Difference between revisions of "Electron microscopy"

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Electron microscopy (view source)

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-This article collection stuffs about '''electron microscopy'''.  It is commonly abbreviated '''EM'''.
+This article is a brief collection things '''electron microscopy'''.  It is commonly abbreviated '''EM'''.
+[[Fixation]] for EM is often done with glutaraldehyde.<ref name=pmid8787969>{{Cite journal  | last1 = Prentø | first1 = P. | title = Glutaraldehyde for electron microscopy: a practical investigation of commercial glutaraldehydes and glutaraldehyde-storage conditions. | journal = Histochem J | volume = 27 | issue = 11 | pages = 906-13 | month = Nov | year = 1995 | doi =  | PMID = 8787969 }}</ref>
+=Ultrastructural findings=
 ==Glycogen==
+===General===
+Seen in:
+*[[Glycogen storage disorders]].
+*[[Ewing sarcoma]].
+===Ultrastructure===
 Appearance:
 *Cytoplasmic, electron dense, not bound by a membrane.
-Seen in:
-*[[Glycogen storage disorders]].
 ==Tubuloreticular inclusions==
 Abbreviated ''TRIs''.
+===General===
+Seen in:
+*[[Dermatomyositis]].
+*[[Inclusion body myositis]].
+===Ultrastructure===
 Appearance:
 *Undulating tubules in the smooth ER, usu. perinuclear.<ref name=pmid19330338>{{cite journal |author=Stoltenburg-Didinger G, Genth E |title=[Dermatomyositis] |language=German |journal=Z Rheumatol |volume=68 |issue=4 |pages=287–94 |year=2009 |month=June |pmid=19330338 |doi=10.1007/s00393-008-0398-y |url=}}</ref>
+==Melanosomes==
+===General===
 Seen in:
-*[[Dermatomyositis]].
+*[[Melanoma]].
-*[[Inclusion body myositis]].
-==Melanosomes==
+===Ultrastructure===
 Appearance:
 *Membrane bound, sausage-like shape.
@@ Line 25: / Line 37: @@
 *[http://www.nature.com/nrm/journal/v8/n10/fig_tab/nrm2258_F1.html Melanosomes (nature.com)].
+==Neurosecretory granules==
+*[[AKA]] ''dense-core granules''.
+===General===
 Seen in:
-*[[Melanoma]].
+*[[Neuroendocrine carcinoma]].
+**[[Merkel cell carcinoma]].<ref name=pmid9062165>{{Cite journal  | last1 = Gil-Moreno | first1 = A. | last2 = Garcia-Jiménez | first2 = A. | last3 = González-Bosquet | first3 = J. | last4 = Esteller | first4 = M. | last5 = Castellví-Vives | first5 = J. | last6 = Martínez Palones | first6 = JM. | last7 = Xercavins | first7 = J. | title = Merkel cell carcinoma of the vulva. | journal = Gynecol Oncol | volume = 64 | issue = 3 | pages = 526-32 | month = Mar | year = 1997 | doi =  | PMID = 9062165 }}</ref>
-==Dense-core granule==
-*[[AKA]] neurosecretory granules
+===Ultrastructure===
 Appearance:<ref>URL: [http://www.pathologyimagesinc.com/emhandbook/diagn-organelles-section/organelle-pages/neuroendo-granules.html http://www.pathologyimagesinc.com/emhandbook/diagn-organelles-section/organelle-pages/neuroendo-granules.html]. Accessed on: 11 February 2011.</ref>
 *Round membrane bound, granular core.
@@ Line 37: / Line 52: @@
 Image(s):
 *[http://pathhsw5m54.ucsf.edu/case7/image77.html Neuroendocrine tumours (ucsf.edu)].
-Seen in:
-*[[Neuroendocrine carcinoma]]s.
 ==Birbeck granules==
+===General===
 *Seen in [[Langerhans cell histiocytosis]].
+===Ultrastructure===
 Appearance:
 *Electron dense, cytoplasmic tennis racket-like body.
@@ Line 51: / Line 65: @@
 **Usually... only the handle of the tennis racket is well seen.
+==Tonofilaments==
+===General===
+*Found in epithelial cells.
+*Part of the cytoskeleton.
+*May be found in the epithelioid component of a biphasic [[synovial sarcoma]].<ref name=pmid9930576>{{Cite journal  | last1 = Fisher | first1 = C. | title = Synovial sarcoma. | journal = Ann Diagn Pathol | volume = 2 | issue = 6 | pages = 401-21 | month = Dec | year = 1998 | doi =  | PMID = 9930576 }}</ref>
+===Ultrastructure===
+Features:
+*Filamentous structures at the cell periphery - vaguely resemble striated muscle.
+Image(s):
+*[http://www.bioscience.org/atlases/tumpath/em/tonofila.htm Tonofilaments (bioscience.org)].
+==Zymogen granules==
+===General===
+*Seen in [[acinic cell carcinoma]].
+*[[Pancreas]] - acinar cell.<ref name=pmid11030603>{{Cite journal  | last1 = Gorelick | first1 = FS. | last2 = Otani | first2 = T. | title = Mechanisms of intracellular zymogen activation. | journal = Baillieres Best Pract Res Clin Gastroenterol | volume = 13 | issue = 2 | pages = 227-40 | month = Jul | year = 1999 | doi =  | PMID = 11030603 }}
+</ref>
+===Ultrastructure===
+Features:
+*Round structures ~0.5-1.5 μm.
+Images:
+*[http://db2.photoresearchers.com/preview/9C3411.html Zymogen granules (photoresearchers.com)].
+*[http://www3.mpibpc.mpg.de/groups/em/Gallery/Pnacreas/158wt1.jpg Zymogen granules (mpg.de)].<ref>URL: [http://www3.mpibpc.mpg.de/groups/em/Gallery.htm http://www3.mpibpc.mpg.de/groups/em/Gallery.htm]. Accessed on: 1 June 2012.</ref>
+=Specific diagnoses=
 ==Malignant mesothelioma==
 {{Main|Malignant mesothelioma}}
@@ Line 57: / Line 99: @@
 *Quantity of intermediate filaments -- mesothelioma have more.
-==See also==
+=See also=
 *[[Microscope]].
 *[[Medical kidney diseases]].
-==References==
+=References=
-{{reflist}}
+{{reflist|2}}
-==External links==
+=External links=
 *[http://www.uni-mainz.de/FB/Medizin/Anatomie/workshop/EM/EMGlykogenE.html Electron microscopy atlas (uni-mainz.de)].
 [[Category:Weird stuff]]

Difference between revisions of "Electron microscopy"

Electron microscopy (view source)

Revision as of 15:29, 4 February 2014

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