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Difference between revisions of "Immunohistochemical staining"
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Immunohistochemical staining (view source)
Revision as of 17:55, 14 September 2013
, 17:55, 14 September 2013→Theory
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==Theory== | ==Theory== | ||
*Antigen retrieval. | |||
*Antibody binds to the antigen. | *Antibody binds to the antigen. | ||
*Amplification - needed as the signal is usually too weak. | *Amplification - needed as the signal is usually too weak. | ||
=== | ===Signaling systems=== | ||
*Avidin-biotin complex. | *Avidin-biotin complex. | ||
**Older.<ref>{{Cite journal | last1 = Vosse | first1 = BA. | last2 = Seelentag | first2 = W. | last3 = Bachmann | first3 = A. | last4 = Bosman | first4 = FT. | last5 = Yan | first5 = P. | title = Background staining of visualization systems in immunohistochemistry: comparison of the Avidin-Biotin Complex system and the EnVision+ system. | journal = Appl Immunohistochem Mol Morphol | volume = 15 | issue = 1 | pages = 103-7 | month = Mar | year = 2007 | doi = | PMID = 17536316 }}</ref> | **Older.<ref>{{Cite journal | last1 = Vosse | first1 = BA. | last2 = Seelentag | first2 = W. | last3 = Bachmann | first3 = A. | last4 = Bosman | first4 = FT. | last5 = Yan | first5 = P. | title = Background staining of visualization systems in immunohistochemistry: comparison of the Avidin-Biotin Complex system and the EnVision+ system. | journal = Appl Immunohistochem Mol Morphol | volume = 15 | issue = 1 | pages = 103-7 | month = Mar | year = 2007 | doi = | PMID = 17536316 }}</ref> | ||
**May suffer from endogenous avidin-biotin activity.<ref name=pmid17536316>{{Cite journal | last1 = Vosse | first1 = BA. | last2 = Seelentag | first2 = W. | last3 = Bachmann | first3 = A. | last4 = Bosman | first4 = FT. | last5 = Yan | first5 = P. | title = Background staining of visualization systems in immunohistochemistry: comparison of the Avidin-Biotin Complex system and the EnVision+ system. | journal = Appl Immunohistochem Mol Morphol | volume = 15 | issue = 1 | pages = 103-7 | month = Mar | year = 2007 | doi = | PMID = 17536316 }}</ref> | **May suffer from endogenous avidin-biotin activity.<ref name=pmid17536316>{{Cite journal | last1 = Vosse | first1 = BA. | last2 = Seelentag | first2 = W. | last3 = Bachmann | first3 = A. | last4 = Bosman | first4 = FT. | last5 = Yan | first5 = P. | title = Background staining of visualization systems in immunohistochemistry: comparison of the Avidin-Biotin Complex system and the EnVision+ system. | journal = Appl Immunohistochem Mol Morphol | volume = 15 | issue = 1 | pages = 103-7 | month = Mar | year = 2007 | doi = | PMID = 17536316 }}</ref> | ||
*Polymer based methods. | *Polymer based methods. | ||
**Newer. | |||
==Quality control== | ==Quality control== | ||