Difference between revisions of "Molecular pathology"

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==Molecular==
==Molecular==
General:
General:
*Very small changes.
*Very small changes - submicroscopic.
*Sequence data.


Techniques:
Techniques:
*DNA sequencing - RT-PCR.
*DNA sequencing.
**Real time-PCR, [[AKA]] real time-quantitative PCR (RQ-PCR).
*RNA sequencing.
*RNA sequencing.
**May be examined after reverse transcription (RNA -> DNA), i.e. RT-PCR.
*Southern blot.
*Southern blot.
**Analysis of proteins.


===Tests===
===Tests===
Line 27: Line 31:
*Large changes (chromosomal).
*Large changes (chromosomal).
**Maximum resolution 3-4 megabase pairs (3-4 million base pairs); may be less - dependent on band density.<ref>{{Ref WMSP|695}}</ref>
**Maximum resolution 3-4 megabase pairs (3-4 million base pairs); may be less - dependent on band density.<ref>{{Ref WMSP|695}}</ref>
*Morphologic data.


Techniques:
Techniques:

Revision as of 18:59, 3 May 2011

Molecular pathology is the future of pathology.

Overview

Molecular pathology can be divided as follows:

 
 
 
Molecular
pathology
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Molecular
techniques
 
 
 
Cytogenetics

Molecular

General:

  • Very small changes - submicroscopic.
  • Sequence data.

Techniques:

  • DNA sequencing.
    • Real time-PCR, AKA real time-quantitative PCR (RQ-PCR).
  • RNA sequencing.
    • May be examined after reverse transcription (RNA -> DNA), i.e. RT-PCR.
  • Southern blot.
    • Analysis of proteins.

Tests

A list of tests are found in the Molecular pathology tests article.

Cytogenetics

General:

  • Large changes (chromosomal).
    • Maximum resolution 3-4 megabase pairs (3-4 million base pairs); may be less - dependent on band density.[1]
  • Morphologic data.

Techniques:

  • ISH = in situ hybridization.
    • FISH = fluorescent in situ hybridization.
    • SISH = silver in situ hybridization.[2]

Image:

World protein databank

I can't help think it is ironic that the protein databank goal is to maintain a free and publicly available archive,[3] yet the announcement is in pay-for-access journal (Nature Structual Biology).[4]

Wnt/beta-catenin pathway

Important in hepatoblastomas.[5]

See also

References

  1. Humphrey, Peter A; Dehner, Louis P; Pfeifer, John D (2008). The Washington Manual of Surgical Pathology (1st ed.). Lippincott Williams & Wilkins. pp. 695. ISBN 978-0781765275.
  2. URL: http://www.immunoportal.com/modules.php?name=News&file=article&sid=186. Accessed on: 2 May 2011.
  3. Worldwide Protein Data Bank. URL: http://www.wwpdb.org/faq.html Accessed on: April 22, 2009.
  4. Berman H, Henrick K, Nakamura H (December 2003). "Announcing the worldwide Protein Data Bank". Nat. Struct. Biol. 10 (12): 980. doi:10.1038/nsb1203-980. PMID 14634627.
  5. Cotran, Ramzi S.; Kumar, Vinay; Fausto, Nelson; Nelso Fausto; Robbins, Stanley L.; Abbas, Abul K. (2005). Robbins and Cotran pathologic basis of disease (7th ed.). St. Louis, Mo: Elsevier Saunders. pp. 923. ISBN 0-7216-0187-1.