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Histology artifacts are common.
- Darker well demarcated line with apparent disruption of the architecture.
- Fine parallel lines.
- Usually due to something intrinsic to the tissue that is hard, e.g. calcium.
- Angulated pieces of tissue with punched-out missing fragments.
- May result in little squares of tissue arranged in a regular pattern.
- Large bubbles of air under the cover slip.
- Black-grey lines - takes on the outline of the tissue beneath it.
- Above the plane-of-focus of the tissue - key feature.