Clinical history
35 year old man, duodenal mass.
Primary image
Low magnification. H&E stain.
Intermediate magnification

Intermediate magnification. H&E stain.

Very high magnification

Very high magnification. H&E stain.

Additional tests
More history
Ask a colleague
Ask a colleague

I wonder if this is syndromic. The guy is sort of young!

Stains
Alcian blue/PAS to Bilirubin 

Test  Result 
Alcian blue/PAS  Dr T would ask why! 
Alican blue pH 1.0  Dr T would ask why! 
Alcian blue pH 2.5  Dr T would ask why! 
Auramine  Dr T would ask why! 
Bielchowsky  Dr T would ask why! 
Bilirubin  Dr T would ask why! 



Colloidal iron to FontanaMasson 

Test  Result 
Colloidal iron  Dr T would ask why! 
Congo red  Dr T would ask why! 
Cresyl violet  Dr T would ask why! 
Dieterle  Dr T would ask why! 
Diff Quik  Dr T would ask why! 
FontanaMasson  Dr T would ask why! 



Gallyas to Gremelius 

Test  Result 
Gallyas  Dr T would ask why! 
Giemsa  Dr T would ask why! 
GMS  Dr T would ask why! 
Gomori's trichrome  Dr T would ask why! 
Gram  Dr T would ask why! 
Gremelius  Dr T would ask why! 



JMS to Mucicarmine 

Test  Result 
JMS  Dr T would ask why! 
Jones  Dr T would ask why! 
Kinyoun  Dr T would ask why! 
Luxol fast blue  Dr T would ask why! 
Masson trichrome  Dr T would ask why! 
MMAS  Dr T would ask why! 
Movat  Dr T would ask why! 
Mucicarmine  Dr T would ask why! 



Oil red O to Prussian blue 

Test  Result 
Oil red O  Dr T would ask why! 
Orecein  Dr T would ask why! 
PAS  Dr T would ask why! 
PASD  Dr T would ask why! 
PASF  Dr T would ask why! 
PTAH  Dr T would ask why! 
Prussian blue  Dr T would ask why! 



Reticulin to ZiehlNeelsen 

Test  Result 
Reticulin  Dr T would ask why! 
Sudan black B  Dr T would ask why! 
Toluidine blue  Dr T would ask why! 
Verhoeff  Dr T would ask why! 
Von Kossa  Dr T would ask why! 
WarthinStarry  Dr T would ask why! 
ZiehlNeeslen  Dr T would ask why! 



IHC
Alpha1 AT to Cathepsin K 

Test  Result 
alpha1 AT  Dr Torres would ask why! 
ACTH  Dr Torres would ask why! 
AE1/AE1  NEGATIVE 
alphafetoprotein  Dr Torres would ask why! 
AlkI  Dr Torres would ask why! 
AMACR  Dr Torres would ask why! 
AR  Dr Torres would ask why! 
ATRX  Dr Torres would ask why! 
Beta2microglobulin  Dr Torres would ask why! 
B72.3  Dr Torres would ask why! 
Betacatenin  Dr Torres would ask why! 
BCL2  Dr Torres would ask why! 
BCL6  Dr Torres would ask why! 
BCLxL  Dr Torres would ask why! 
C3 comp  Dr Torres would ask why! 
CA9  Dr Torres would ask why! 
Calcitonin  Dr Torres would ask why! 
Calponin  Dr Torres would ask why! 
Calretinin  Dr Torres would ask why! 
CAM5.2  Dr Torres would ask why! 
Cathepsin K  Dr Torres would ask why! 



CD10 to Chromogranin 

Test  Result 
CD10  Dr Torres would ask why! 
CD117  Dr Torres would ask why! 
CD138  Dr Torres would ask why! 
CD15  Dr Torres would ask why! 
CD1a  Dr Torres would ask why! 
CD20  Dr Torres would ask why! 
CD21  Dr Torres would ask why! 
CD23  Dr Torres would ask why! 
CD3  Dr Torres would ask why! 
CD30  Dr Torres would ask why! 
CD31  Dr Torres would ask why! 
CD34  Dr Torres would ask why! 
CD35  Dr Torres would ask why! 
CD4  Dr Torres would ask why! 
CD43  Dr Torres would ask why! 
CD45 (LCA)  Dr Torres would ask why! 
CD5  Dr Torres would ask why! 
CD56  POSITIVE 
CD57  Dr Torres would ask why! 
CD68  Dr Torres would ask why! 
CD7  Dr Torres would ask why! 
CD79a  Dr Torres would ask why! 
CD8  Dr Torres would ask why! 
CD99  Dr Torres would ask why! 
CDX2  Dr Torres would ask why! 
CEAm  Dr Torres would ask why! 
Chromogranin  



CK17 to Glypican 3 

Test  Result 
CK17  Dr Torres would ask why! 
CK19  Dr Torres would ask why! 
CK20  Dr Torres would ask why! 
CK34betaE12  Dr Torres would ask why! 
CK5/6  Dr Torres would ask why! 
CK7  Dr Torres would ask why! 
CMV  Dr Torres would ask why! 
cMYC  Dr Torres would ask why! 
Cyclin D1  Dr Torres would ask why! 
D240  Dr Torres would ask why! 
Desmin  Dr Torres would ask why! 
DOG1  Dr Torres would ask why! 
EBV  Dr Torres would ask why! 
EMA  NEGATIVE 
ER and PR  Dr Torres would ask why! 
Factor VIII  Dr Torres would ask why! 
Factor XIIIa  Dr Torres would ask why! 
Fascin  Dr Torres would ask why! 
FH  Dr Torres would ask why! 
FSH  Dr Torres would ask why! 
Gastrin  Dr Torres would ask why! 
GATA3  Dr Torres would ask why! 
GCDFP15 (BRST2)  Dr Torres would ask why! 
GFAP  Dr Torres would ask why! 
GH  Dr Torres would ask why! 
Glucagon  Dr Torres would ask why! 
Glypican3  Dr Torres would ask why! 



HBME1 to IgM 

Test  Result 
HBME1  Dr Torres would ask why! 
HBV core  Dr Torres would ask why! 
HBV surface  Dr Torres would ask why! 
Hcaldesmon  Dr Torres would ask why! 
HCG  Dr Torres would ask why! 
Helicobacter  Dr Torres would ask why! 
Hepatocyte  Dr Torres would ask why! 
HER2/neu  Dr Torres would ask why! 
HHV8  Dr Torres would ask why! 
HMB45  Dr Torres would ask why! 
HNF1beta  Dr Torres would ask why! 
HPV  Dr Torres would ask why! 
HSVI  Dr Torres would ask why! 
HSVII  Dr Torres would ask why! 
IDH1  Dr Torres would ask why! 
Inhibin  Dr Torres would ask why! 
INI1 (BAF47)  Dr Torres would ask why! 
Insulin  Dr Torres would ask why! 
Kappa  Dr Torres would ask why! 
Ki67  Dr Torres would ask why! 
Lambda  Dr Torres would ask why! 
Leu 7  Dr Torres would ask why! 
IgA  Dr Torres would ask why! 
IgG  Dr Torres would ask why! 
IgM  Dr Torres would ask why! 



LH to PDGFR 

Test  Result 
LH  Dr Torres would ask why! 
LIN28  Dr Torres would ask why! 
Lysozyme  Dr Torres would ask why! 
mammoglobin  Dr Torres would ask why! 
MAP2  Dr Torres would ask why! 
MCV  Dr Torres would ask why! 
Melanin A  Dr Torres would ask why! 
MHC class I  Dr Torres would ask why! 
MITF  Dr Torres would ask why! 
MUM1  Dr Torres would ask why! 
Myeloperoxidase  Dr Torres would ask why! 
MYO D1  Dr Torres would ask why! 
Myoglobin  Dr Torres would ask why! 
Napsin  Dr Torres would ask why! 
NF  Dr Torres would ask why! 
NKX3.1  Dr Torres would ask why! 
NSE  POSITIVE 
OCT3/4  Dr Torres would ask why! 
p16  Dr Torres would ask why! 
P501S  Dr Torres would ask why! 
p53  Dr Torres would ask why! 
p57  Dr Torres would ask why! 
p63  Dr Torres would ask why! 
Pankeratin  NEGATIVE 
PAX2  Dr Torres would ask why! 
PAX5  Dr Torres would ask why! 
PAX8  Dr Torres would ask why! 
PCNA  Dr Torres would ask why! 
PDGFR  Dr Torres would ask why! 



PLAP to WT1 

Test  Result 
PLAP  Dr Torres would ask why! 
PNL2C  Dr Torres would ask why! 
Prolactin  Dr Torres would ask why! 
PSA  Dr Torres would ask why! 
PSAP  Dr Torres would ask why! 
RCC  Dr Torres would ask why! 
S100  
SALL4  Dr Torres would ask why! 
Smooth muscle actin  Dr Torres would ask why! 
Somatostatin  Dr Torres would ask why! 
STAT6  Dr Torres would ask why! 
Synaptophysin  POSITIVE 
TdT  Dr Torres would ask why! 
TFE3  Dr Torres would ask why! 
TFEB  Dr Torres would ask why! 
Thyroglobulin  Dr Torres would ask why! 
Toxoplasma  Dr Torres would ask why! 
TSH  Dr Torres would ask why! 
TTF1  Dr Torres would ask why! 
Ubiquitin  Dr Torres would ask why! 
UCHL1 (PGP9.5)  Dr Torres would ask why! 
Ulex Europaeus  Dr Torres would ask why! 
Vimentin  Dr Torres would ask why! 
VIP  Dr Torres would ask why! 
VZV  Dr Torres would ask why! 
WT1  Dr Torres would ask why! 



Differential diagnosisDiagnosisOther cases